Abstract
Purpose
HO-1 has been proved to be associated with tumor aggressivity and poor prognosis in various cancers. Our study provides the first study to demonstrate the relationship of HO-1 expression and clinical characteristics in ovarian cancer patients.
Methods
Immunohistochemistry and western blotting were used to examine the expression of HO-1 in tissue species and fresh tissues. CCK-8 was used to investigate cell viability. Transwell chamber was performed to estimate migration and invasion capacities in A2780 and Skov-3 cells.
Results
Immunohistochemistry and western blotting showed that the expression of HO-1 was higher in ovarian cancer tissues than normal ovarian tissues. High expression of HO-1 was significantly associated with serous ovarian cancer, high FIGO stage, lymph node metastasis, and non-optimal debulking. Patients with high expression of HO-1 exhibited an unfavorable prognosis. In vitro inducing the expression of HO-1 promoted the proliferation and metastasis of A2780 and Skov-3 cells, with the increased expressions of mesenchymal marker (Vimentin), epithelial–mesenchymal transition-associated transcript factor (Zeb-1), anti-apoptotic protein (Bcl-2), and the decreased expressions of epithelial marker (Keratin) and pro-apoptotic protein (Bax). Meanwhile, after incubating A2780 and Skov-3 together with HO-1 inhibitor, above results could be reversed.
Conclusion
HO-1 might be a potential marker for prediction of ovarian cancer prognosis and a target for ovarian cancer treatment.
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Change history
20 October 2017
n the original version of the article, Table 2 was incorrect. The corrected Table 2 is shown here. Therefore, in Results (page 3 of original version, right column, line 13), the OR of non-optimal debulking should read OR = 3.036 with 95% CI 1.452–6.348.
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Acknowledgements
This work was supported by grants from the Development and Reform commission Project of Shandong Province (26010104081103).
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A correction to this article is available online at https://doi.org/10.1007/s12094-017-1769-0.
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Zhao, Z., Xu, Y., Lu, J. et al. High expression of HO-1 predicts poor prognosis of ovarian cancer patients and promotes proliferation and aggressiveness of ovarian cancer cells. Clin Transl Oncol 20, 491–499 (2018). https://doi.org/10.1007/s12094-017-1738-7
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DOI: https://doi.org/10.1007/s12094-017-1738-7