Inhibition of Glioblastoma Growth by the Thiadiazolidinone Compound TDZD-8
Figure 2
Histological and immunohistochemical analysis of tumors induced by GL261 glioblastoma cells.
(A) Representative hematoxylin-eosin stained sections of tumors at 12 and 24 days after injection. Insets show higher magnifications of images shown in the upper panels. Tumors derived from control animals showed clear mixoid matrices (arrows), multinucleated malignant and pleomorphic cells (asterisks), and numerous mitotic figures (arrowheads). (B) Immunohistochemical study of tumor sections for PCNA and active caspase-3 detection 12 days after injection of GL261 cells. Insets show a higher magnification of the images shown in the upper panels. Scale bars, 300 µm. Insets scale bars, 25 µm. Quantification of the number of active caspase-3 and PCNA positive cells was evaluated in tumor sections and values expressed as mean ± SD. positive cells/field. ***p≤0.001.