doi: 10.1016/s0014-5793(00)01820-2.
Membrane topology of the N-terminus of the Escherichia coli FtsK division protein
Affiliations
- PMID: 10922461
- DOI: 10.1016/s0014-5793(00)01820-2
Item in Clipboard
Membrane topology of the N-terminus of the Escherichia coli FtsK division protein
FEBS Lett.
.
Free article
Abstract
The Escherichia coli FtsK protein targets the septum, is essential for cell division and may play a role in DNA partitioning. Computer modelling suggests that the first 180 amino acids of the protein are embedded in the cytoplasmic membrane by up to six transmembrane domains. We demonstrate, using gene fusions, that the N-terminus contains four transmembrane helices that link two periplasmic domains. The first periplasmic domain contains an HEXXH amino acid sequence characteristic of zinc metalloproteases. We show by mutation analysis that the conserved glutamic acid of the HEXXH sequence is essential for FtsK function during septation.
Similar articles
-
Biogenesis of inner membrane proteins in Escherichia coli.Mol Microbiol. 2001 Apr;40(2):314-22. doi: 10.1046/j.1365-2958.2001.02392.x. Mol Microbiol. 2001. PMID: 11309115 Review.
-
Membrane topology of the Mep/Amt family of ammonium transporters.Mol Microbiol. 2000 Jul;37(2):331-44. doi: 10.1046/j.1365-2958.2000.01994.x. Mol Microbiol. 2000. PMID: 10931328
-
Escherichia coli DipZ: anatomy of a transmembrane protein disulphide reductase in which three pairs of cysteine residues, one in each of three domains, contribute differentially to function.Mol Microbiol. 2000 Mar;35(6):1360-74. doi: 10.1046/j.1365-2958.2000.01796.x. Mol Microbiol. 2000. PMID: 10760137
-
Use of phoA and lacZ fusions to study the membrane topology of ProW, a component of the osmoregulated ProU transport system of Escherichia coli.J Bacteriol. 1996 Sep;178(18):5370-81. doi: 10.1128/jb.178.18.5370-5381.1996. J Bacteriol. 1996. PMID: 8808924 Free PMC article.
-
Analysis of membrane protein topology using alkaline phosphatase and beta-galactosidase gene fusions.Methods Cell Biol. 1991;34:61-75. doi: 10.1016/s0091-679x(08)61676-3. Methods Cell Biol. 1991. PMID: 1943817 Review. No abstract available.
Cited by
-
The Essential Genome of Escherichia coli K-12.mBio. 2018 Feb 20;9(1):e02096-17. doi: 10.1128/mBio.02096-17. mBio. 2018. PMID: 29463657 Free PMC article.
-
Site-directed fluorescence labeling reveals a revised N-terminal membrane topology and functional periplasmic residues in the Escherichia coli cell division protein FtsK.J Biol Chem. 2014 Aug 22;289(34):23287-301. doi: 10.1074/jbc.M114.569624. Epub 2014 Jul 7. J Biol Chem. 2014. PMID: 25002583 Free PMC article.
-
Separating speed and ability to displace roadblocks during DNA translocation by FtsK.EMBO J. 2010 Apr 21;29(8):1423-33. doi: 10.1038/emboj.2010.29. Epub 2010 Apr 8. EMBO J. 2010. PMID: 20379135 Free PMC article.
-
Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK.EMBO J. 2010 Feb 3;29(3):597-605. doi: 10.1038/emboj.2009.381. Epub 2009 Dec 24. EMBO J. 2010. PMID: 20033058 Free PMC article.
-
ATP-binding site lesions in FtsE impair cell division.J Bacteriol. 2009 Jun;191(12):3772-84. doi: 10.1128/JB.00179-09. Epub 2009 Apr 17. J Bacteriol. 2009. PMID: 19376877 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
