Suppression of adhesion and invasion of hepatoma cells in culture by tea compounds through antioxidative activity
- PMID: 10996728
- DOI: 10.1016/s0304-3835(00)00545-0
Suppression of adhesion and invasion of hepatoma cells in culture by tea compounds through antioxidative activity
Abstract
To determine the actions of tea components on the invasion of a rat ascites hepatoma cell line of AH109A and to understand their modes of action, the cancer cells were co-cultured with a rat mesentery-derived mesothelial cell monolayer in the presence of tea components. The synergistic effects of (-)-epicatechin (EC) with (-)-epigallocatechin gallate (EGCG) on AH109A invasion were demonstrated. Further study showed that 10 microM of EGCG or theaflavins, or 2.5 microM of ethylenediaminetetra-acetic (EDTA) entirely abolished the increase in AH109A adhesion and invasion stimulated by reactive oxygen species (ROS) from the hypoxanthine-xanthine oxidase system. Our results suggest that (.)OH(-)- and other ROS-scavenging activity of EGCG and theaflavins may be responsible for the inhibition of (.)OH(-)- and related ROS-potentiated AH109A adhesion and invasion to the cultured rat mesothelial cell monolayer.
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