Fermentations of fructo-oligosaccharides and their components by Bifidobacterium infantis ATCC 15697 on batch culture in semi-synthetic medium
- PMID: 11412315
- DOI: 10.1046/j.1365-2672.2001.01317.x
Fermentations of fructo-oligosaccharides and their components by Bifidobacterium infantis ATCC 15697 on batch culture in semi-synthetic medium
Abstract
Aims: To compare the physiological behaviour of Bifidobacterium infantis ATCC 15697 growing on synthetic oligofructose or its components.
Methods and results: The studies were carried out in regulated or non-regulated batch cultures on semi-synthetic media. Differences between the carbohydrate utilization patterns with glucose, fructose, sucrose and fructo-oligosaccharides (FOS) were determined. Glucose was the preferred substrate for growth and biomass production, whereas fructose was the best for lactate and acetate production. With sucrose, biomass production reached the level obtained with glucose, whereas with FOS, more metabolites were produced, as with fructose. In a mixture of FOS, the shorter saccharides were used first and fructose was released in the medium. Fructofuranosidase, an enzyme necessary to hydrolyse FOS, was inducible by fructose.
Conclusion: Glucose contained in FOS and sucrose might sustain growth and cell production, while fructose might enable the production of major metabolites.
Significance and impact of the study: A better understanding of the bifidogenic nature of oligofructose has been gained.
Similar articles
-
New Trends and Technological Challenges in the Industrial Production and Purification of Fructo-oligosaccharides.Crit Rev Food Sci Nutr. 2015;55(10):1444-55. doi: 10.1080/10408398.2012.697082. Crit Rev Food Sci Nutr. 2015. PMID: 24915327 Review.
-
In vitro kinetic analysis of oligofructose consumption by Bacteroides and Bifidobacterium spp. indicates different degradation mechanisms.Appl Environ Microbiol. 2006 Feb;72(2):1006-12. doi: 10.1128/AEM.72.2.1006-1012.2006. Appl Environ Microbiol. 2006. PMID: 16461642 Free PMC article.
-
Kinetics of Bifidobacterium longum ATCC 15707 fermentations: effect of the dilution rate and carbon source.Appl Microbiol Biotechnol. 2005 Jun;67(5):623-30. doi: 10.1007/s00253-004-1792-2. Epub 2005 Mar 4. Appl Microbiol Biotechnol. 2005. PMID: 15747118
-
The bifidogenic nature of chicory inulin and its hydrolysis products.J Nutr. 1998 Jan;128(1):11-9. doi: 10.1093/jn/128.1.11. J Nutr. 1998. PMID: 9430596 Review.
-
Enrichment of bifidobacteria from human gut contents by oligofructose using continuous culture.FEMS Microbiol Lett. 1994 May 1;118(1-2):121-7. doi: 10.1111/j.1574-6968.1994.tb06813.x. FEMS Microbiol Lett. 1994. PMID: 8013867
Cited by
-
Growth and survival of Bifidobacterium breve and Bifidobacterium longum in various sugar systems with fructooligosaccharide supplementation.J Food Sci Technol. 2022 Oct;59(10):3775-3786. doi: 10.1007/s13197-022-05361-z. Epub 2022 Jan 20. J Food Sci Technol. 2022. PMID: 36193365 Free PMC article.
-
Intake of MPRO3 over 4 Weeks Reduces Glucose Levels and Improves Gastrointestinal Health and Metabolism.Microorganisms. 2021 Dec 31;10(1):88. doi: 10.3390/microorganisms10010088. Microorganisms. 2021. PMID: 35056536 Free PMC article.
-
Probiotics, prebiotics and synbiotics: Safe options for next-generation therapeutics.Appl Microbiol Biotechnol. 2022 Jan;106(2):505-521. doi: 10.1007/s00253-021-11646-8. Epub 2022 Jan 11. Appl Microbiol Biotechnol. 2022. PMID: 35015145 Free PMC article. Review.
-
Identifying the essential nutritional requirements of the probiotic bacteria Bifidobacterium animalis and Bifidobacterium longum through genome-scale modeling.NPJ Syst Biol Appl. 2021 Dec 9;7(1):47. doi: 10.1038/s41540-021-00207-4. NPJ Syst Biol Appl. 2021. PMID: 34887435 Free PMC article.
-
Effects of Fructose and Oligofructose Addition on Milk Fermentation Using Novel Lactobacillus Cultures to Obtain High-Quality Yogurt-like Products.Molecules. 2021 Sep 22;26(19):5730. doi: 10.3390/molecules26195730. Molecules. 2021. PMID: 34641276 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
