Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Sep;127(1):222-9.
doi: 10.1104/pp.127.1.222.

Overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in Arabidopsis

J K Branen  1 , T J Chiou, N J Engeseth
Affiliations

Overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in Arabidopsis

J K Branen et al. Plant Physiol. 2001 Sep.

Abstract

Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesis has been established, the role of the diverse number of isoforms has yet to be elucidated. We have generated transgenic Arabidopsis plants that express high levels of ACP-1, a seed-predominant ACP isoform, in leaf tissue under control of the cauliflower mosaic virus 35S promoter. Western and northern analysis of these plants demonstrate 3- to 8-fold increased expression of this isoform in leaf tissue, but no significant changes in seed. Analysis of the fatty acid composition of leaf tissue revealed that overexpression of ACP-1 in leaf tissue alters fatty acid composition. Significant decreases in levels of 16:3 were noted along with increases in 18:3. These findings represent the first in vivo report that overexpression of an ACP isoform results in changes in fatty acid composition in plants.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Design of the binary transformation vector number 6 in pGA748. BL, Left border of Ti plasmid; NPTII, neomycin phosphotransferase gene; CaMV 35S; ACP-1, SalI/BamHI fragment of the Arabidopsis ACP-1 genomic clone (pAD4) in 3′-5′ (antisense) conformation; 400+-bp region, the region upstream from transcription start-site of ACP-1; BR, right border of Ti plasmid.
Figure 2
Figure 2
Western blot of ACP proteins in Arabidopsis whole leaf tissue. Lane 1, WT; lanes 2 and 3, transgenic control pGA748; lanes 4 through 11, independent transgenic number 6 plants; lane 12, ACP-1 protein; lane 13, ACP-2 protein. LMI, Leaf major isoform.
Figure 3
Figure 3
Percentages (based on WT amount) of ACP-1 sense mRNA in whole leaf RNA. PS, Transgenic control; 6c, 6d, and 6g, independent transgenic number 6 plants. Values were normalized using β-actin as a control for equal loading.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. An G, Ebert PR, Mitra A, Ha SB. Binary Vectors. In: Gelvin SP, Schilperoort RA, editors. Plant Molecular Biology Manual, Chapter A3. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1988. pp. 1–19.
    1. Baerson SR, Vander Heiden MG, Lamppa GK. Identification of domains in an Arabidopsis acyl carrier protein gene promoter required for maximal organ-specific expression. Plant Mol Biol. 1994;26:1947–1959. - PubMed
    1. Battey JF, Ohlrogge JB. Evolutionary and tissue-specific control of expression of multiple acyl-carrier protein isoforms in plants and bacteria. Planta. 1990;180:352–360. - PubMed
    1. Bechtold N, Ellis J, Pelletier G. In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Paris Life Sci. 1993;316:1194–1199.
    1. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959;37:911–917. - PubMed

Publication types

LinkOut - more resources

-