Automatic identification of proteins with a MALDI-quadrupole ion trap mass spectrometer
- PMID: 11721901
- DOI: 10.1021/ac010682o
Automatic identification of proteins with a MALDI-quadrupole ion trap mass spectrometer
Abstract
A matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer of new design is described. The instrument is based on a commercial Finnegan LCQ ion trap mass spectrometer to which we have added a MALDI ion source that incorporates a sample stage constructed from a compact disk and a new ion transmission interface. The ion interface contains a quadrupole ion guide installed between the skimmer and the octapoles of the original instrument configuration, allowing for operation in both MALDI and electrospray ionization modes. The instrument has femtomole sensitivity for peptides and is capable of collecting a large number of MALDI MS and MALDI MS/MS spectra within a short period of time. The MALDI source produces reproducible signals for 10(4)-10(5) laser pulses, enabling us to collect MS/MS spectra from all the discernible singly charged ions detected in a MS peptide map. We describe the different modes of the instrument operation and algorithms for data processing as applied to challenging protein identification problems.
Similar articles
-
Dual-source mass spectrometer with MALDI-LIT-ESI configuration.J Proteome Res. 2007 Feb;6(2):837-45. doi: 10.1021/pr060514i. J Proteome Res. 2007. PMID: 17269740
-
Hybrid quadrupole/time-of-flight mass spectrometers for analysis of biomolecules.Methods Enzymol. 2005;402:49-78. doi: 10.1016/S0076-6879(05)02002-1. Methods Enzymol. 2005. PMID: 16401506 Review.
-
Determination of glycopeptide structures by multistage mass spectrometry with low-energy collision-induced dissociation: comparison of electrospray ionization quadrupole ion trap and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron time-of-flight approaches.Rapid Commun Mass Spectrom. 2004;18(14):1575-82. doi: 10.1002/rcm.1521. Rapid Commun Mass Spectrom. 2004. PMID: 15282782
-
Analysis of high mass peptides using a novel matrix-assisted laser desorption/ionisation quadrupole ion trap time-of-flight mass spectrometer.Rapid Commun Mass Spectrom. 2003;17(12):1358-1365. doi: 10.1002/rcm.1044. Rapid Commun Mass Spectrom. 2003. PMID: 12811760
-
The nature of collision-induced dissociation processes of doubly protonated peptides: comparative study for the future use of matrix-assisted laser desorption/ionization on a hybrid quadrupole time-of-flight mass spectrometer in proteomics.Rapid Commun Mass Spectrom. 2001;15(22):2058-66. doi: 10.1002/rcm.485. Rapid Commun Mass Spectrom. 2001. PMID: 11746869
Cited by
-
FolC2-mediated folate metabolism contributes to suppression of inflammation by probiotic Lactobacillus reuteri.Microbiologyopen. 2016 Oct;5(5):802-818. doi: 10.1002/mbo3.371. Epub 2016 Jun 28. Microbiologyopen. 2016. PMID: 27353144 Free PMC article.
-
Discovery and characterisation of a novel toxin from Dendroaspis angusticeps, named Tx7335, that activates the potassium channel KcsA.Sci Rep. 2016 Apr 5;6:23904. doi: 10.1038/srep23904. Sci Rep. 2016. PMID: 27044983 Free PMC article.
-
Phosphorylation of CEACAM1 molecule by calmodulin kinase IID in a three-dimensional model of mammary gland lumen formation.J Biol Chem. 2014 Jan 31;289(5):2934-45. doi: 10.1074/jbc.M113.496992. Epub 2013 Dec 3. J Biol Chem. 2014. PMID: 24302721 Free PMC article.
-
Rif1 prevents resection of DNA breaks and promotes immunoglobulin class switching.Science. 2013 Feb 8;339(6120):711-5. doi: 10.1126/science.1230624. Epub 2013 Jan 10. Science. 2013. PMID: 23306439 Free PMC article.
-
High-Capacity Ion Trap Coupled to a Time-of-Flight Mass Spectrometer for Comprehensive Linked Scans with no Scanning Losses.Int J Mass Spectrom. 2011 Mar 30;301(1-3):211-219. doi: 10.1016/j.ijms.2010.09.013. Int J Mass Spectrom. 2011. PMID: 21516228 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources