Is there a relationship between the supramolecular organization of the mitochondrial ATP synthase and the formation of cristae?
- PMID: 12206911
- DOI: 10.1016/s0005-2728(02)00274-8
Is there a relationship between the supramolecular organization of the mitochondrial ATP synthase and the formation of cristae?
Abstract
Blue native polyacrylamide gel electrophoresis (BN-PAGE) analyses of detergent mitochondrial extracts have provided evidence that the yeast ATP synthase could form dimers. Cross-linking experiments performed on a modified version of the i-subunit of this enzyme indicate the existence of such ATP synthase dimers in the yeast inner mitochondrial membrane. We also show that the first transmembrane segment of the eukaryotic b-subunit (bTM1), like the two supernumerary subunits e and g, is required for dimerization/oligomerization of ATP synthases. Unlike mitochondria of wild-type cells that display a well-developed cristae network, mitochondria of yeast cells devoid of subunits e, g, or bTM1 present morphological alterations with an abnormal proliferation of the inner mitochondrial membrane. From these observations, we postulate that an anomalous organization of the inner mitochondrial membrane occurs due to the absence of ATP synthase dimers/oligomers. We provide a model in which the mitochondrial ATP synthase is a key element in cristae morphogenesis.
Similar articles
-
ATP synthase oligomerization: from the enzyme models to the mitochondrial morphology.Int J Biochem Cell Biol. 2013 Jan;45(1):99-105. doi: 10.1016/j.biocel.2012.05.017. Epub 2012 Jun 1. Int J Biochem Cell Biol. 2013. PMID: 22664329 Review.
-
Mitochondrial F1F0-ATP synthase and organellar internal architecture.Int J Biochem Cell Biol. 2009 Oct;41(10):1783-9. doi: 10.1016/j.biocel.2009.01.011. Epub 2009 Jan 24. Int J Biochem Cell Biol. 2009. PMID: 19703649 Review.
-
The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits.Biochemistry. 2006 May 30;45(21):6715-23. doi: 10.1021/bi0601407. Biochemistry. 2006. PMID: 16716082
-
The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane.Eur J Biochem. 2003 Apr;270(8):1875-84. doi: 10.1046/j.1432-1033.2003.03557.x. Eur J Biochem. 2003. PMID: 12694201
-
The ATP synthase is involved in generating mitochondrial cristae morphology.EMBO J. 2002 Feb 1;21(3):221-30. doi: 10.1093/emboj/21.3.221. EMBO J. 2002. PMID: 11823415 Free PMC article.
Cited by
-
ATP synthase FOF1 structure, function, and structure-based drug design.Cell Mol Life Sci. 2022 Mar 6;79(3):179. doi: 10.1007/s00018-022-04153-0. Cell Mol Life Sci. 2022. PMID: 35253091 Free PMC article. Review.
-
Pathways shaping the mitochondrial inner membrane.Open Biol. 2021 Dec;11(12):210238. doi: 10.1098/rsob.210238. Epub 2021 Dec 1. Open Biol. 2021. PMID: 34847778 Free PMC article. Review.
-
Inhibition of the mitochondrial ATPase function by IF1 changes the spatiotemporal organization of ATP synthase.Biochim Biophys Acta Bioenerg. 2021 Jan 1;1862(1):148322. doi: 10.1016/j.bbabio.2020.148322. Epub 2020 Oct 14. Biochim Biophys Acta Bioenerg. 2021. PMID: 33065099 Free PMC article.
-
Protonic Capacitor: Elucidating the biological significance of mitochondrial cristae formation.Sci Rep. 2020 Jun 29;10(1):10304. doi: 10.1038/s41598-020-66203-6. Sci Rep. 2020. PMID: 32601276 Free PMC article.
-
Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart.Elife. 2019 Dec 23;8:e50163. doi: 10.7554/eLife.50163. Elife. 2019. PMID: 31868166 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
