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. 2003 Mar 28;303(1):69-73.
doi: 10.1016/s0006-291x(03)00309-7.

ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis

Junya Masumoto  1 , Theresa A Dowds, Philip Schaner, Felicia F Chen, Yasunori Ogura, Mu Li, Li Zhu, Tsutomu Katsuyama, Junji Sagara, Shun'ichiro Taniguchi, Deborah L Gumucio, Gabriel Núñez, Naohiro Inohara
Affiliations

ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis

Junya Masumoto et al. Biochem Biophys Res Commun. .

Abstract

ASC is a pro-apoptotic protein containing a pyrin domain (PD) and a caspase-recruitment domain (CARD). A previous study suggests that ASC interacts with Ipaf, a member of the Apaf-1/Nod1 protein family. However, the functional relevance of the interaction has not been determined. Here, we report that co-expression of ASC with Ipaf or oligomerization of ASC induces both apoptosis and NF-kappa B activation. Apoptosis induced through ASC was inhibited by a mutant form of Caspase-8 but not by that of Caspase-1. The PD of ASC physically interacted with Caspase-8 as well as with pyrin, the familial Mediterranean fever gene product. Caspase-8 deficiency rescued mouse fibroblasts from apoptosis induced by ASC oligomerization. Pyrin disrupted the interaction between ASC and Caspase-8, and inhibited both apoptosis and NF-kappa B activation induced by ASC. These findings suggest that ASC is a mediator of NF-kappa B activation and Caspase-8-dependent apoptosis in an Ipaf signaling pathway.

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