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. 2005 Mar;12(3):474-6.
doi: 10.1128/CDLI.12.3.474-476.2005.

Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus

Maofeng Qiu  1 , Jin Wang, Hongxia Wang, Zeliang Chen, Erhei Dai, Zhaobiao Guo, Xiaoyi Wang, Xin Pang, Baoxing Fan, Jie Wen, Jian Wang, Ruifu Yang
Affiliations

Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus

Maofeng Qiu et al. Clin Diagn Lab Immunol. 2005 Mar.

Abstract

Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

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Figures

FIG. 1.
FIG. 1.
Detection of antibodies to SARS-CoV by the N13-based antigen-capturing ELISA, the N-based ELISA, and the indirect ELISA based on whole-virus lysate in one twofold serially diluted serum sample from a SARS patient. The cutoff OD450 values for these assays were 0.17, 0.17, and 0.18, respectively.
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