Dose-response study of probiotic bacteria Bifidobacterium animalis subsp lactis BB-12 and Lactobacillus paracasei subsp paracasei CRL-341 in healthy young adults
- PMID: 16721394
- DOI: 10.1038/sj.ejcn.1602450
Dose-response study of probiotic bacteria Bifidobacterium animalis subsp lactis BB-12 and Lactobacillus paracasei subsp paracasei CRL-341 in healthy young adults
Abstract
Objective: This study was performed to investigate the dose-response effects of supplementation with Bifidobacterium animalis subsp lactis (BB-12) and Lactobacillus paracasei subsp paracasei (CRL-431) on blood lipids, recovery from feces and bowel habits. Changes of the fecal microflora was analyzed in the 10(10) CFU/day probiotic and placebo group.
Design: The study was designed as a randomized, placebo-controlled, double-blinded, parallel dose-response study.
Subjects: Healthy young adults (18-40 years) were recruited by advertising in local newspapers. Of the 75 persons enrolled, 71 (46 women, 25 men, mean age 25.6 years (range 18-40 years)) completed the study.
Intervention: The volunteers were randomly assigned into five groups receiving either placebo or a mixture of the two probiotics in the concentration of 10(8), 10(9), 10(10) or 10(11) CFU/day in 2 weeks run-in period, 3 weeks intervention and 2 weeks wash-out. Diary reporting bowel habits and well being (abdominal bloating, flatulence and headache) was kept for all 7 weeks and blood lipids, fecal recovery of BB-12 and CRL-431, as well as fecal microflora was tested before, immediately and 2 weeks after intervention.
Results: The fecal recovery of BB-12 increased significantly (P < 0.001) with increasing dose. In the group receiving 10(11) CFU/day BB-12 was recovered from 13 out of 15 volunteers. CRL-431 was not recovered in any of the fecal samples. Supplementation with probiotics did not change the fecal bacterial composition. A significant linear increase in fecal consistency (looser stool) with increasing probiotic dose (P = 0.018) was observed. No overall dose-response effect was found on the blood lipids. High doses of probiotics were well tolerated.
Conclusion: A dose-related recovery of BB-12 from feces was observed.
Similar articles
-
To add or not to add probiotics to infant formulae? An updated systematic review.Benef Microbes. 2017 Oct 13;8(5):717-725. doi: 10.3920/BM2016.0233. Epub 2017 Aug 31. Benef Microbes. 2017. PMID: 28856907 Review.
-
Alterations in fecal microbiota composition by probiotic supplementation in healthy adults: a systematic review of randomized controlled trials.Genome Med. 2016 May 10;8(1):52. doi: 10.1186/s13073-016-0300-5. Genome Med. 2016. PMID: 27159972 Free PMC article. Review.
-
Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults.Eur J Clin Nutr. 2008 May;62(5):584-93. doi: 10.1038/sj.ejcn.1602761. Epub 2007 Apr 18. Eur J Clin Nutr. 2008. PMID: 17440520 Clinical Trial.
-
Impact of consumption of different levels of Bifidobacterium lactis HN019 on the intestinal microflora of elderly human subjects.J Nutr Health Aging. 2007 Jan-Feb;11(1):26-31. J Nutr Health Aging. 2007. PMID: 17315077 Clinical Trial.
-
Immunomodulating potential of supplementation with probiotics: a dose-response study in healthy young adults.FEMS Immunol Med Microbiol. 2006 Aug;47(3):380-90. doi: 10.1111/j.1574-695X.2006.00109.x. FEMS Immunol Med Microbiol. 2006. PMID: 16872374 Clinical Trial.
Cited by
-
Effect of Probiotic Dose Escalation on Gut Microbiota and Clinical Outcomes in Preterm Infants-A Systematic Review.Children (Basel). 2023 Oct 20;10(10):1710. doi: 10.3390/children10101710. Children (Basel). 2023. PMID: 37892373 Free PMC article. Review.
-
The Different Ways Multi-Strain Probiotics with Different Ratios of Bifidobacterium and Lactobacillus Relieve Constipation Induced by Loperamide in Mice.Nutrients. 2023 Sep 30;15(19):4230. doi: 10.3390/nu15194230. Nutrients. 2023. PMID: 37836514 Free PMC article.
-
Exploring the Dose-Effect Relationship of Bifidobacterium longum in Relieving Loperamide Hydrochloride-Induced Constipation in Rats through Colon-Released Capsules.Int J Mol Sci. 2023 Apr 1;24(7):6585. doi: 10.3390/ijms24076585. Int J Mol Sci. 2023. PMID: 37047557 Free PMC article.
-
Effect of Probiotic Supplementation on Gut Microbiota in Patients with Major Depressive Disorders: A Systematic Review.Nutrients. 2023 Mar 10;15(6):1351. doi: 10.3390/nu15061351. Nutrients. 2023. PMID: 36986088 Free PMC article. Review.
-
Bifidobacterium animalis subsp. lactis HN019 Effects on Gut Health: A Review.Front Nutr. 2021 Dec 14;8:790561. doi: 10.3389/fnut.2021.790561. eCollection 2021. Front Nutr. 2021. PMID: 34970580 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources