Induction of CYP1A by green tea extract in human intestinal cell lines
- PMID: 16773535
- DOI: 10.1055/s-2006-931537
Induction of CYP1A by green tea extract in human intestinal cell lines
Abstract
In this study the influence of green tea extract (GTE) or its component epigallocatechin gallate (EGCG) on the expression of different cytochrome P450 (CYP) isoenzymes was investigated in the human gastrointestinal epithelial cell lines LS-180 and Caco-2. Additionally, the effect of GTE or EGCG on functional activity of different CYP isoenzymes was investigated in vitro. mRNA expression levels were determined by quantitative RT-PCR and compared with protein levels. In LS-180 cells GTE, but not EGCG, significantly induced CYP1A2 mRNA expression, whereas neither CYP1A1 nor CYP3A4 mRNA expression was modulated by GTE or EGCG. In Caco-2 cells CYP1A1 as well as CYP1A2 mRNA expression was significantly increased in a dose-dependent manner by GTE and EGCG. However, EGCG alone was about 3-5-fold less effective than GTE. mRNA expression of CYP1A1 or CYP1A2 induced by the promutagen benzo[a]pyrene was significantly down-regulated by EGCG but not by GTE. CYP1A protein levels in response to GTE in Caco-2 and LS-180 cells confirmed the mRNA expression results. CYP activity was measured with CYP1A2 or CYP3A4 expressed in insect cell membranes using a luminescent method. GTE or EGCG significantly inhibited CYP1A2 and CYP3A4 function in a dose-dependent manner. Therefore, it appears that green tea moderately modulates the expression of drug-metabolizing enzymes but non-specifically inhibits the function of human CYPs. Since CYP enzymes play an important role in detoxification processes, these results might be of relevance for the prediction of the outcome of future clinical studies.
Similar articles
-
New cancer treatment strategy using combination of green tea catechins and anticancer drugs.Cancer Sci. 2011 Feb;102(2):317-23. doi: 10.1111/j.1349-7006.2010.01805.x. Epub 2010 Dec 30. Cancer Sci. 2011. PMID: 21199169 Review.
-
[Effects of green tea extract on expression of human papillomavirus type 16 oncoproteins-induced hypoxia-inducible factor-1alpha and vascular endothelial growth factor in human cervical carcinoma cells].Zhonghua Yi Xue Za Zhi. 2008 Nov 4;88(40):2872-7. Zhonghua Yi Xue Za Zhi. 2008. PMID: 19080502 Chinese.
-
Proposed mechanisms of (-)-epigallocatechin-3-gallate for anti-obesity.Chem Biol Interact. 2007 Apr 25;167(2):85-98. doi: 10.1016/j.cbi.2007.02.008. Epub 2007 Feb 20. Chem Biol Interact. 2007. PMID: 17368440 Review.
-
Green tea extract induces interleukin-8 (IL-8) mRNA and protein expression but specifically inhibits IL-8 secretion in caco-2 cells.Planta Med. 2006 Jun;72(8):697-702. doi: 10.1055/s-2006-931597. Epub 2006 May 31. Planta Med. 2006. PMID: 16739069
-
Cytochrome P450 expression and activities in human tongue cells and their modulation by green tea extract.Toxicol Appl Pharmacol. 2005 Jan 15;202(2):140-50. doi: 10.1016/j.taap.2004.06.014. Toxicol Appl Pharmacol. 2005. PMID: 15629189
Cited by
-
The Influence of EGCG on the Pharmacokinetics and Pharmacodynamics of Bisoprolol and a New Method for Simultaneous Determination of EGCG and Bisoprolol in Rat Plasma.Front Nutr. 2022 May 31;9:907986. doi: 10.3389/fnut.2022.907986. eCollection 2022. Front Nutr. 2022. PMID: 35711541 Free PMC article.
-
The effect of grape seed and green tea extracts on the pharmacokinetics of imatinib and its main metabolite, N-desmethyl imatinib, in rats.BMC Pharmacol Toxicol. 2020 Nov 16;21(1):77. doi: 10.1186/s40360-020-00456-9. BMC Pharmacol Toxicol. 2020. PMID: 33198812 Free PMC article.
-
Antioxidants for Healthy Skin: The Emerging Role of Aryl Hydrocarbon Receptors and Nuclear Factor-Erythroid 2-Related Factor-2.Nutrients. 2017 Mar 3;9(3):223. doi: 10.3390/nu9030223. Nutrients. 2017. PMID: 28273792 Free PMC article. Review.
-
Variable inhibitory effect of herbal supplements of different brands on human P450 CYP1A2.EXCLI J. 2012 Feb 2;11:7-19. eCollection 2012. EXCLI J. 2012. PMID: 27298605 Free PMC article.
-
Pharmacokinetic Interactions between Drugs and Botanical Dietary Supplements.Drug Metab Dispos. 2016 Feb;44(2):162-71. doi: 10.1124/dmd.115.066902. Epub 2015 Oct 5. Drug Metab Dispos. 2016. PMID: 26438626 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical