Phenotypic analysis of IL-10-treated, monocyte-derived dendritic cells in patients with systemic lupus erythematosus
- PMID: 17083624
- DOI: 10.1111/j.1365-3083.2006.01849.x
Phenotypic analysis of IL-10-treated, monocyte-derived dendritic cells in patients with systemic lupus erythematosus
Abstract
Dendritic cells (DC) play a dual role in the immune response, participating in its induction, and the maintenance of immune tolerance. The aim of this work was to perform a quantitative and phenotypic analysis of DC generated in vitro in the presence of IL-10 in patients with systemic lupus erythematosus (SLE). Blood samples were obtained from 10 active and untreated patients with SLE and six controls. Monocyte-derived DC were generated in vitro in the presence or absence of IL-10, and a quantitative and phenotypic analysis was performed. We found that freshly isolated monocytes from SLE patients had an increased expression of CD11b. On the other hand, the efficiency of in vitro DC generation was diminished in blood samples from SLE patients for conventional DC, but not for IL-10-treated DC. A diminished expression of HLA-DR, CD9 and CD86 was observed in conventional DC from SLE patients compared with controls. In contrast, enhanced levels of HLA-DR, CD80, CD9 and CD151 tetraspanins, FN1 (a class II MHC-tetraspanin epitope), CD85j/ILT2 and CD69 were detected in IL-10-treated DC from SLE patients. Accordingly, the phenotypic profile of IL-10-treated DC was very different in SLE and controls. However, the synthesis of IL-10 and IL-12 was similar in IL-10-treated and conventional cells in both SLE patients and controls. Our findings on the aberrant phenotype of IL-10-treated DC in SLE and their normal efficiency of in vitro generation may be important for the design of future therapies of this condition based on the administration of DC to induce immune tolerance.
Similar articles
-
Altered dendritic cells with tolerizing phenotype in patients with systemic lupus erythematosus.Eur J Immunol. 2008 Oct;38(10):2896-904. doi: 10.1002/eji.200838342. Eur J Immunol. 2008. PMID: 18958888
-
Prolactin, dendritic cells, and systemic lupus erythematosus.Autoimmun Rev. 2008 Jan;7(3):251-5. doi: 10.1016/j.autrev.2007.11.018. Epub 2007 Dec 3. Autoimmun Rev. 2008. PMID: 18190887 Review.
-
Monocyte-derived dendritic cells from chronic HCV patients are not infected but show an immature phenotype and aberrant cytokine profile.Liver Int. 2007 Sep;27(7):944-53. doi: 10.1111/j.1478-3231.2007.01507.x. Liver Int. 2007. PMID: 17696933
-
The role myeloid dendritic cells play in the pathogenesis of systemic lupus erythematosus.Autoimmun Rev. 2007 Aug;6(7):450-6. doi: 10.1016/j.autrev.2007.01.014. Epub 2007 Feb 23. Autoimmun Rev. 2007. PMID: 17643932 Review.
-
Alterations of dendritic cells in systemic lupus erythematosus: phenotypic and functional deficiencies.Arthritis Rheum. 2001 Apr;44(4):856-65. doi: 10.1002/1529-0131(200104)44:4<856::AID-ANR142>3.0.CO;2-A. Arthritis Rheum. 2001. PMID: 11315925
Cited by
-
Human leukocyte immunoglobulin-like receptors in health and disease.Front Immunol. 2023 Nov 13;14:1282874. doi: 10.3389/fimmu.2023.1282874. eCollection 2023. Front Immunol. 2023. PMID: 38022598 Free PMC article. Review.
-
Increased ILT2+ natural killer T cells correlate with disease activity in systemic lupus erythematosus.Clin Rheumatol. 2023 Nov;42(11):3113-3121. doi: 10.1007/s10067-023-06750-1. Epub 2023 Sep 11. Clin Rheumatol. 2023. PMID: 37695380
-
Immunoglobulin-like receptors and the generation of innate immune memory.Immunogenetics. 2022 Feb;74(1):179-195. doi: 10.1007/s00251-021-01240-7. Epub 2022 Jan 16. Immunogenetics. 2022. PMID: 35034136 Free PMC article. Review.
-
Leukocyte Immunoglobulin-Like Receptors in Regulating the Immune Response in Infectious Diseases: A Window of Opportunity to Pathogen Persistence and a Sound Target in Therapeutics.Front Immunol. 2021 Sep 14;12:717998. doi: 10.3389/fimmu.2021.717998. eCollection 2021. Front Immunol. 2021. PMID: 34594332 Free PMC article. Review.
-
Regulatory Dendritic Cells, T Cell Tolerance, and Dendritic Cell Therapy for Immunologic Disease.Front Immunol. 2021 Mar 10;12:633436. doi: 10.3389/fimmu.2021.633436. eCollection 2021. Front Immunol. 2021. PMID: 33777019 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous