Organotin speciation and tissue distribution in rat dams, fetuses, and neonates following oral administration of tributyltin chloride
- PMID: 18246498
- DOI: 10.1080/15287390701801653
Organotin speciation and tissue distribution in rat dams, fetuses, and neonates following oral administration of tributyltin chloride
Abstract
Tributyltin (TBT) is a biocide that contaminates human foodstuffs, especially shellfish. TBT is an endocrine disrupter, producing imposex in several marine gastropods. Previous studies showed that oral dosing of rat dams with TBT chloride leads to abnormal fetal and postnatal development. In this study, the tissue distribution and speciation of organotins in tissues were examined in dams, fetuses, and neonates following dosing of rat dams commencing on gestational day (GD) 8 by oral gavage with TBT in olive oil at 0, 0.25, 2.5, or 10 mg/kg body weight (BW)/d. Dams' body weights were significantly reduced by the 10-mg/kg BW/d TBT treatment. At GD20, there were no significant effects of any TBT treatment on pup weights, litter size, sex ratio, or tissue weights. However, at postnatal day (PND) 6 and 12, neonatal pup weights were reduced by the 10-mg/kg BW/d TBT treatment but tissue weights were unaffected, except for the liver weight of female pups, which was reduced by the 10-mg/kg BW/d TBT treatment. Tissues harvested on GD20 and PND6 and PND12 were extracted for determination of organotins by gas chromatography-atomic emission detection (GC-AED). In most tissues, TBT and its metabolite dibutyltin (DBT) were evident but monobutyltin (MBT) was rarely measured above the detection limit. The livers and brains of fetuses contained TBT and DBT at levels that were approximately 50% of the equivalent tissues in the dams. Furthermore, these tissues appeared to preferentially absorb/retain organotins, since the concentrations were greater than were found for the total loading in whole pups. The placenta also contained relatively large quantities of TBT and DBT. Postnatally, the TBT levels in pups decreased markedly, a probable consequence of the extremely low levels of organotins in rat milk. However, DBT levels in pups livers and brains were maintained, probably due to metabolism of TBT to DBT. Similarly, while dams' spleens contained significant quantities of organotins, the pups' spleens contained smaller quantities, and these decreased rapidly between PND6 and PND12. These results show that organotins cross the placenta and accumulate in fetal tissues but that during lactation, the pups would receive minimal organotins through the milk and during this period, the levels of TBT in pups' tissues decreases rapidly. Consequently, fetuses would be at greater risk of the adverse effects of TBT, but due to the lack of transfer through milk, the risk would be reduced during the lactational period.
Similar articles
-
Human exposure, biomarkers, and fate of organotins in the environment.Rev Environ Contam Toxicol. 2011;213:27-54. doi: 10.1007/978-1-4419-9860-6_2. Rev Environ Contam Toxicol. 2011. PMID: 21541847 Review.
-
Endocrine disruption induced by organotin compounds; organotins function as a powerful agonist for nuclear receptors rather than an aromatase inhibitor.J Toxicol Sci. 2008 Aug;33(3):269-76. doi: 10.2131/jts.33.269. J Toxicol Sci. 2008. PMID: 18670157 Review.
-
Oral (gavage), in utero and postnatal exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part 1: Toxicology, histopathology and clinical chemistry.Food Chem Toxicol. 2004 Feb;42(2):211-20. doi: 10.1016/j.fct.2003.09.003. Food Chem Toxicol. 2004. PMID: 14667468
-
Effects of in utero tributyltin chloride exposure in the rat on pregnancy outcome.Toxicol Sci. 2003 Aug;74(2):407-15. doi: 10.1093/toxsci/kfg131. Epub 2003 May 28. Toxicol Sci. 2003. PMID: 12773765
-
NTP technical report on the toxicity studies of Dibutyl Phthalate (CAS No. 84-74-2) Administered in Feed to F344/N Rats and B6C3F1 Mice.Toxic Rep Ser. 1995 Apr;30:1-G5. Toxic Rep Ser. 1995. PMID: 12209194
Cited by
-
Toxic Relationships: Prediction of TBT's Affinity to the Ecdysteroid Receptor of Triops longicaudatus.Toxics. 2023 Nov 17;11(11):937. doi: 10.3390/toxics11110937. Toxics. 2023. PMID: 37999589 Free PMC article.
-
Organotin Antifouling Compounds and Sex-Steroid Nuclear Receptor Perturbation: Some Structural Insights.Toxics. 2022 Dec 27;11(1):25. doi: 10.3390/toxics11010025. Toxics. 2022. PMID: 36668751 Free PMC article.
-
Perinatal exposure to tributyltin affects feeding behavior and expression of hypothalamic neuropeptide Y in the paraventricular nucleus of adult mice.J Anat. 2023 Feb;242(2):235-244. doi: 10.1111/joa.13766. Epub 2022 Sep 8. J Anat. 2023. PMID: 36073672 Free PMC article.
-
Evaluation of Fetal Exposures to Metals and Metalloids through Meconium Analyses: A Review.Int J Environ Res Public Health. 2021 Feb 18;18(4):1975. doi: 10.3390/ijerph18041975. Int J Environ Res Public Health. 2021. PMID: 33670707 Free PMC article. Review.
-
Effects of exposure to triphenyltin (TPT) contaminant on sperm activity in adulthood of Calomys laucha exposed through breastfeeding.Environ Sci Pollut Res Int. 2019 Mar;26(8):8280-8288. doi: 10.1007/s11356-019-04365-1. Epub 2019 Jan 31. Environ Sci Pollut Res Int. 2019. PMID: 30706268
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous