Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid
- PMID: 1854329
- PMCID: PMC1151201
- DOI: 10.1042/bj2770133
Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid
Abstract
Cigarette smoke (CS) is known to contain a large number of oxidants. In order to assess the oxidative effects of CS on biological fluids, we exposed human blood plasma to filtered (gas phase) and unfiltered (whole) CS, and determined the rate of utilization of endogenous antioxidants in relation to the appearance of lipid hydroperoxides. Lipid peroxidation was measured with a specific and sensitive assay that can detect lipid hydroperoxides at plasma levels as low as 10 nM. We found that exposure of plasma to the gas phase of CS, but not to whole CS, induces lipid peroxidation once endogenous ascorbic acid has been oxidized completely. In addition, CS exposure caused oxidation of plasma protein thiols and albumin-bound bilirubin, whereas uric acid and alpha-tocopherol were not consumed at significant rates. In plasma exposed to the gas phase of CS, low-density lipoprotein exhibited slightly increased electrophoretic mobility, but there was no apparent degradation of apolipoprotein B. Our results support the concept of an increased vitamin C utilization in smokers, and suggest that lipid peroxidation induced by oxidants present in the gas phase of CS leads to potentially atherogenic changes in lipoproteins.
Similar articles
-
Smoking, oxidative stress and cardiovascular diseases--do anti-oxidative therapies fail?Curr Med Chem. 2007;14(16):1703-12. doi: 10.2174/092986707781058959. Curr Med Chem. 2007. PMID: 17627508 Review.
-
A critical overview of the chemistry of copper-dependent low density lipoprotein oxidation: roles of lipid hydroperoxides, alpha-tocopherol, thiols, and ceruloplasmin.Arch Biochem Biophys. 2001 Oct 1;394(1):117-35. doi: 10.1006/abbi.2001.2509. Arch Biochem Biophys. 2001. PMID: 11566034 Review.
-
Vitamin C prevents cigarette smoke induced oxidative damage of proteins and increased proteolysis.Free Radic Biol Med. 1999 Nov;27(9-10):1064-79. doi: 10.1016/s0891-5849(99)00154-9. Free Radic Biol Med. 1999. PMID: 10569639
-
Cigarette smoke oxidation of human plasma constituents.Ann N Y Acad Sci. 1993 May 28;686:72-89; discussion 89-90. doi: 10.1111/j.1749-6632.1993.tb39157.x. Ann N Y Acad Sci. 1993. PMID: 8512263
-
Ascorbic acid protects lipids in human plasma and low-density lipoprotein against oxidative damage.Am J Clin Nutr. 1991 Dec;54(6 Suppl):1113S-1118S. doi: 10.1093/ajcn/54.6.1113s. Am J Clin Nutr. 1991. PMID: 1962556
Cited by
-
Metal mixture exposures and serum lipid levels in childhood: the Rhea mother-child cohort in Greece.J Expo Sci Environ Epidemiol. 2024 May 2. doi: 10.1038/s41370-024-00674-x. Online ahead of print. J Expo Sci Environ Epidemiol. 2024. PMID: 38698271
-
Decrease in Sperm Parameters in the 21st Century: Obesity, Lifestyle, or Environmental Factors? An Updated Narrative Review.J Pers Med. 2024 Feb 11;14(2):198. doi: 10.3390/jpm14020198. J Pers Med. 2024. PMID: 38392631 Free PMC article. Review.
-
GC-MS and GC-MS/MS measurement of malondialdehyde (MDA) in clinical studies: Pre-analytical and clinical considerations.J Mass Spectrom Adv Clin Lab. 2023 Aug 5;30:10-24. doi: 10.1016/j.jmsacl.2023.08.001. eCollection 2023 Nov. J Mass Spectrom Adv Clin Lab. 2023. PMID: 37637438 Free PMC article. Review.
-
Aging, oxidative stress and degenerative diseases: mechanisms, complications and emerging therapeutic strategies.Biogerontology. 2023 Oct;24(5):609-662. doi: 10.1007/s10522-023-10050-1. Epub 2023 Jul 30. Biogerontology. 2023. PMID: 37516673 Review.
-
Tobacco smoking and vascular biology and function: evidence from human studies.Pflugers Arch. 2023 Jul;475(7):797-805. doi: 10.1007/s00424-023-02805-z. Epub 2023 Mar 24. Pflugers Arch. 2023. PMID: 36961561 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical