Coadministration of active phthalates results in disruption of foetal testicular function in rats
- PMID: 19207615
- DOI: 10.1111/j.1365-2605.2008.00939.x
Coadministration of active phthalates results in disruption of foetal testicular function in rats
Abstract
The reproductive effects of the coadministration of di-2-(ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) were studied in both foetal and adult male rat offspring exposed in utero. Pregnant Wistar rats were treated by oral gavage from gestation day 13 to 21 with vehicle control, 150 mg DEHP/kg body weight (bw)/day, 100 mg DBP/kg bw/ or a combination of the two compounds (DEHP 150 + DBP 100 mg/kg bw/day). An additional group of dams received 500 mg DBP/kg bw/day. A significant decrease in foetal testicular testosterone levels was observed in animals exposed to 500 mg DBP/kg/day or the phthalate mixture. Similarly, histological analysis of the foetal testis revealed that the coadministration of DEHP and DBP was able to increase the diameter of seminiferous cords and induce gonocyte multinucleation at doses that individually had no significant effects on these variables. However, in the phthalate mixture group, no significant changes were observed in anogenital distance and nipple retention, variables that are used to indicate possible anti-androgenic effects. Also, the adult endpoints investigated, that included reproductive organ weights and the number of spermatids per testis, were unaffected by any treatment regimen. Overall, coadministration of DEHP and DBP in utero significantly reduced testicular testosterone levels and resulted in misshapen seminiferous cords and gonocyte multinucleation in rat foetal testis. Our results also confirm that these foetal endpoints seem to be the most sensitive markers of prenatal phthalate exposure.
Similar articles
-
Reproductive and developmental toxicity of phthalates.J Toxicol Environ Health B Crit Rev. 2009 Apr;12(4):225-49. doi: 10.1080/10937400903094091. J Toxicol Environ Health B Crit Rev. 2009. PMID: 20183522 Review.
-
A dose response study following in utero and lactational exposure to di-(2-ethylhexyl) phthalate (DEHP): reproductive effects on adult male offspring rats.Toxicology. 2006 Nov 10;228(1):85-97. doi: 10.1016/j.tox.2006.08.020. Epub 2006 Aug 22. Toxicology. 2006. PMID: 16996189
-
A dose-response study following in utero and lactational exposure to di-(2-ethylhexyl) phthalate (DEHP): effects on androgenic status, developmental landmarks and testicular histology in male offspring rats.Toxicology. 2006 Aug 1;225(1):64-74. doi: 10.1016/j.tox.2006.05.007. Epub 2006 May 19. Toxicology. 2006. PMID: 16806631
-
Disruption of reproductive development in male rat offspring following in utero exposure to phthalate esters.Int J Androl. 2006 Feb;29(1):140-7; discussion 181-5. doi: 10.1111/j.1365-2605.2005.00563.x. Epub 2005 Aug 11. Int J Androl. 2006. PMID: 16102138 Review.
-
NTP technical report on the toxicity studies of Dibutyl Phthalate (CAS No. 84-74-2) Administered in Feed to F344/N Rats and B6C3F1 Mice.Toxic Rep Ser. 1995 Apr;30:1-G5. Toxic Rep Ser. 1995. PMID: 12209194
Cited by
-
On the Use and Interpretation of Areola/Nipple Retention as a Biomarker for Anti-androgenic Effects in Rat Toxicity Studies.Front Toxicol. 2021 Oct 27;3:730752. doi: 10.3389/ftox.2021.730752. eCollection 2021. Front Toxicol. 2021. PMID: 35295101 Free PMC article. Review.
-
Exposure to Dibutyl Phthalate and Reproductive-Related Outcomes in Animal Models: Evidence From Rodents Study.Front Physiol. 2021 Dec 8;12:684532. doi: 10.3389/fphys.2021.684532. eCollection 2021. Front Physiol. 2021. PMID: 34955869 Free PMC article. Review.
-
Endocrine disrupting chemicals: Impact on human health, wildlife and the environment.Sci Prog. 2019 Mar;102(1):3-42. doi: 10.1177/0036850419826802. Epub 2019 Jan 1. Sci Prog. 2019. PMID: 31829784 Free PMC article. Review.
-
Systematic reviews and meta-analyses of human and animal evidence of prenatal diethylhexyl phthalate exposure and changes in male anogenital distance.J Toxicol Environ Health B Crit Rev. 2018;21(4):207-226. doi: 10.1080/10937404.2018.1505354. Epub 2018 Sep 10. J Toxicol Environ Health B Crit Rev. 2018. PMID: 30199328 Free PMC article.
-
A systematic review on the adverse health effects of di-2-ethylhexyl phthalate.Environ Sci Pollut Res Int. 2016 Dec;23(24):24642-24693. doi: 10.1007/s11356-016-7648-3. Epub 2016 Oct 6. Environ Sci Pollut Res Int. 2016. PMID: 27714658 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources