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Randomized Controlled Trial
. 2010 Jun;21(6):544-9.
doi: 10.1016/j.jnutbio.2009.03.002. Epub 2009 May 15.

Supplementation with lutein or lutein plus green tea extracts does not change oxidative stress in adequately nourished older adults

Lei Li  1 , C-Y Oliver Chen, Giancarlo Aldini, Elizabeth J Johnson, Helen Rasmussen, Yasukazu Yoshida, Etsuo Niki, Jeffrey B Blumberg, Robert M Russell, Kyung-Jin Yeum
Affiliations
Randomized Controlled Trial

Supplementation with lutein or lutein plus green tea extracts does not change oxidative stress in adequately nourished older adults

Lei Li et al. J Nutr Biochem. 2010 Jun.

Abstract

Epigallocatechin gallate, a major component of green tea polyphenols, protects against the oxidation of fat-soluble antioxidants including lutein. The current study determined the effect of a relatively high but a dietary achievable dose of lutein or lutein plus green tea extract on antioxidant status. Healthy subjects (50-70 years) were randomly assigned to one of two groups (n=20 in each group): (1) a lutein (12 mg/day) supplemented group or (2) a lutein (12 mg/day) plus green tea extract (200 mg/day) supplemented group. After 2 weeks of run-in period consuming less than two servings of lightly colored fruits and vegetables in their diet, each group was treated for 112 days while on their customary regular diets. Plasma carotenoids including lutein, tocopherols, flavanols and ascorbic acid were analyzed by HPLC-UVD and HPLC-electrochemical detector systems; total antioxidant capacity by fluorometry; lipid peroxidation by malondialdehyde using a HPLC system with a fluorescent detector and by total hydroxyoctadecadienoic acids using a GC/MS. Plasma lutein, total carotenoids and ascorbic acid concentrations of subjects in either the lutein group or the lutein plus green tea extract group were significantly increased (P<.05) at 4 weeks and throughout the 16-week study period. However, no significant changes from baseline in any biomarker of overall antioxidant activity or lipid peroxidation of the subjects were seen in either group. Our results indicate that an increase of antioxidant concentrations within a range that could readily be achieved in a healthful diet does not affect in vivo antioxidant status in normal healthy subjects when sufficient amounts of antioxidants already exist.

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Figures

Fig 1
Fig 1. Plasma fat-soluble antioxidant concentrations over time in older adults (aged 50–70 y) supplemented with either lutein (n=20) or lutein + green tea extract (n=20) for 112d
The lutein group (L) supplemented with 12 mg/d lutein and the lutein + green tea extract group (L+G) supplemented with 12 mg/d lutein and 200 mg/d green tea extract. A: Lutein, B: total carotenoid (lutein + β-carotene + lycopene), C: α-tocopherol The data are expressed as mean ± SD. One-way repeated measures ANOVA with Tukey multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with a Tukey multiple comparison test was used. Significantly different as compared with the baseline *P<0.05, **p<0.01. Samples were analyzed by HPLC with UV detection for carotenoids and tocopherol.
Fig 2
Fig 2. Plasma water-soluble antioxidant concentrations over time in older adults (aged 50–70 y) supplemented with either lutein (n=20) or lutein + green tea extract (n=20) for 112d
The lutein group (L) supplemented with 12 mg/d lutein and the lutein + green tea extract group (L+G) supplemented with 12 mg/d lutein and 200 mg/d green tea extract. The data are expressed as mean ± SD. One-way repeated measures ANOVA with Tukey multiple comparison was performed. When an equal variance test failed, Friedman repeated measures analysis of variance on ranks with a Tukey multiple comparison test was used. Significantly different as compared with the baseline *P<0.05. Samples were analyzed by HPLC with electrochemical detection for ascorbic acid.
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