Flow injection-capillary electrophoresis frontal analysis method for the study of the interactions of a series of drugs with human serum albumin
- PMID: 19695968
- DOI: 10.1016/j.jchromb.2009.08.002
Flow injection-capillary electrophoresis frontal analysis method for the study of the interactions of a series of drugs with human serum albumin
Abstract
In this paper, a fast method for the study on the interactions of a series of drugs used in the treatment of hypertension with human serum albumin (HSA) by flow injection-capillary electrophoresis (FI-CE) was developed based on the principle of frontal analysis (FA). The binding parameters were determined by FI-CE-FA from Scatchard equation and compared with results obtained by non-CE methods and literature values. A multiple linear regression (MLR) model between the drug-protein binding constants (K) and structural descriptors of drugs was constructed. L-tryptophan (L-try) and phenylbutazone (PB) were used as displacement reagents to investigate the binding sites of a series of drugs on HSA. The binding synergism effect between drugs and the effects of many metal ions existing in human plasma on protein binding were also investigated systematically.
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