The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria
- PMID: 19820708
- DOI: 10.1038/ni.1800
The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria
Abstract
Cell-autonomous innate immune responses against bacteria attempting to colonize the cytosol of mammalian cells are incompletely understood. Polyubiquitylated proteins can accumulate on the surface of such bacteria, and bacterial growth is restricted by Tank-binding kinase (TBK1). Here we show that NDP52, not previously known to contribute to innate immunity, recognizes ubiquitin-coated Salmonella enterica in human cells and, by binding the adaptor proteins Nap1 and Sintbad, recruits TBK1. Knockdown of NDP52 and TBK1 facilitated bacterial proliferation and increased the number of cells containing ubiquitin-coated salmonella. NDP52 also recruited LC3, an autophagosomal marker, and knockdown of NDP52 impaired autophagy of salmonella. We conclude that human cells utilize the ubiquitin system and NDP52 to activate autophagy against bacteria attempting to colonize their cytosol.
Comment in
-
NDP52: the missing link between ubiquitinated bacteria and autophagy.Nat Immunol. 2009 Nov;10(11):1137-9. doi: 10.1038/ni1109-1137. Nat Immunol. 2009. PMID: 19841643 Free PMC article.
Similar articles
-
TBK1 mediates crosstalk between the innate immune response and autophagy.Sci Signal. 2011 Aug 9;4(187):pe39. doi: 10.1126/scisignal.2002355. Sci Signal. 2011. PMID: 21868362 Review.
-
Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth.Science. 2011 Jul 8;333(6039):228-33. doi: 10.1126/science.1205405. Epub 2011 May 26. Science. 2011. PMID: 21617041 Free PMC article.
-
How cells deploy ubiquitin and autophagy to defend their cytosol from bacterial invasion.Autophagy. 2011 Mar;7(3):304-9. doi: 10.4161/auto.7.3.14539. Autophagy. 2011. PMID: 21193841 Review.
-
NDP52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria.Autophagy. 2010 Feb;6(2):288-9. doi: 10.4161/auto.6.2.11118. Epub 2010 Feb 4. Autophagy. 2010. PMID: 20104023
-
NDP52: the missing link between ubiquitinated bacteria and autophagy.Nat Immunol. 2009 Nov;10(11):1137-9. doi: 10.1038/ni1109-1137. Nat Immunol. 2009. PMID: 19841643 Free PMC article.
Cited by
-
Vaccinia virus subverts xenophagy through phosphorylation and nuclear targeting of p62.J Cell Biol. 2024 Jun 3;223(6):e202104129. doi: 10.1083/jcb.202104129. Epub 2024 May 6. J Cell Biol. 2024. PMID: 38709216 Free PMC article.
-
Friends and Foes: The Ambivalent Role of Autophagy in HIV-1 Infection.Viruses. 2024 Mar 25;16(4):500. doi: 10.3390/v16040500. Viruses. 2024. PMID: 38675843 Free PMC article. Review.
-
Identification of autophagy receptors for the Crohn's disease-associated adherent-invasive Escherichia coli.Front Cell Infect Microbiol. 2024 Mar 28;14:1268243. doi: 10.3389/fcimb.2024.1268243. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38606299 Free PMC article.
-
Interplay between group A Streptococcus and host innate immune responses.Microbiol Mol Biol Rev. 2024 Mar 27;88(1):e0005222. doi: 10.1128/mmbr.00052-22. Epub 2024 Mar 7. Microbiol Mol Biol Rev. 2024. PMID: 38451081 Review.
-
Mechanistic insights into the interactions of TAX1BP1 with RB1CC1 and mammalian ATG8 family proteins.Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2315550121. doi: 10.1073/pnas.2315550121. Epub 2024 Mar 4. Proc Natl Acad Sci U S A. 2024. PMID: 38437556
References
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous