NDP52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria
- PMID: 20104023
- DOI: 10.4161/auto.6.2.11118
NDP52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria
Abstract
Autophagy functions as a cell-autonomous effector mechanism of innate immunity by separating bacteria from cytosolic resources and delivering them for lysosomal destruction. How cytosolic bacteria are targeted for autophagy is incompletely understood. We recently discovered that Salmonella enterica serotype Typhimurium and Streptococcus pyogenes are detected by NDP52 (nuclear dot protein 52 kDa), after these bacteria enter the cytosol of human cells and become decorated with polyubiquitinated proteins. NDP52 binds the bacterial ubiquitin coat as well as ATG8/LC3 and delivers cytosolic bacteria into autophagosomes. In the absence of NDP52 ubiquitin-coated bacteria accumulate outside ATG8/LC3(+) autophagosomes. Cells lacking NDP52 fail to restrict bacterial proliferation, as do cells depleted of TBK1, an IKK family kinase colocalizing with NDP52 at the bacterial surface. Our findings demonstrate the existence of a receptor for the selective autophagy of cytosolic bacteria, suggesting that cells are able to differentiate between antibacterial and other forms of autophagy.
Similar articles
-
The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria.Nat Immunol. 2009 Nov;10(11):1215-21. doi: 10.1038/ni.1800. Epub 2009 Oct 11. Nat Immunol. 2009. PMID: 19820708
-
An essential role for the ATG8 ortholog LC3C in antibacterial autophagy.Autophagy. 2013 May;9(5):784-6. doi: 10.4161/auto.23698. Epub 2013 Feb 22. Autophagy. 2013. PMID: 23434839 Free PMC article.
-
How cells deploy ubiquitin and autophagy to defend their cytosol from bacterial invasion.Autophagy. 2011 Mar;7(3):304-9. doi: 10.4161/auto.7.3.14539. Autophagy. 2011. PMID: 21193841 Review.
-
Dual function of CALCOCO2/NDP52 during xenophagy.Autophagy. 2015;11(6):965-6. doi: 10.1080/15548627.2015.1046672. Autophagy. 2015. PMID: 25998689 Free PMC article. Review.
-
The ubiquitin-like modifier FAT10 decorates autophagy-targeted Salmonella and contributes to Salmonella resistance in mice.J Cell Sci. 2014 Nov 15;127(Pt 22):4883-93. doi: 10.1242/jcs.152371. Epub 2014 Sep 30. J Cell Sci. 2014. PMID: 25271057
Cited by
-
Interlinked destinies: How ubiquitin-proteasome and autophagy systems underpin neurocognitive outcomes.Exp Neurol. 2024 Sep;379:114869. doi: 10.1016/j.expneurol.2024.114869. Epub 2024 Jun 18. Exp Neurol. 2024. PMID: 38901755 Review.
-
Identification of autophagy receptors for the Crohn's disease-associated adherent-invasive Escherichia coli.Front Cell Infect Microbiol. 2024 Mar 28;14:1268243. doi: 10.3389/fcimb.2024.1268243. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38606299 Free PMC article.
-
Seneca Valley virus 3C protease cleaves OPTN (optineurin) to Impair selective autophagy and type I interferon signaling.Autophagy. 2024 Mar;20(3):614-628. doi: 10.1080/15548627.2023.2277108. Epub 2023 Nov 6. Autophagy. 2024. PMID: 37930946
-
The ATG8 Family Proteins GABARAP and GABARAPL1 Target Antigen to Dendritic Cells to Prime CD4+ and CD8+ T Cells.Cells. 2022 Sep 6;11(18):2782. doi: 10.3390/cells11182782. Cells. 2022. PMID: 36139357 Free PMC article.
-
A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes.Nat Commun. 2022 Jun 27;13(1):3656. doi: 10.1038/s41467-022-31351-y. Nat Commun. 2022. PMID: 35760786 Free PMC article.
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous