MiR-221 expression affects invasion potential of human prostate carcinoma cell lines by targeting DVL2
- PMID: 21487968
- DOI: 10.1007/s12032-011-9934-8
MiR-221 expression affects invasion potential of human prostate carcinoma cell lines by targeting DVL2
Abstract
The aim of this study is to evaluate the effect of the variation of miR-221 on the prostate cancer cells' NE differentiation and invasive function and to examine the function of miR-221 in plasma as a blood-based miRNA biomarker candidate for CaP. The expression of 7 miRNAs in LNCaP, LNCaP-AI, and PC3 prostate cancer cell lines was detected by Northern blotting. LNCaP and LNCaP-AI cells cultured in androgen-depleted medium were transfected with different synthetic miRs. The ability of invasiveness was evaluated by a Matrigel invasion assay. Cell growth was assessed by using the CCK-8 cell proliferation assay at different times. The expression of NSE and DVL2 during the neuroendocrine phenotype and migration were measured by qRT-PCR and Western blot. The level of miR-221 in the prostate cancer samples was measured by qRT-PCR. MiR-221 was significantly increased compared AIPC with ADPC cell lines. Overexpression of miR-221 in LNCaP cells significantly increased the level of NSE expression and induced NE differentiation. Knocking down the level of miR-221 expression with antagonist miR-221 in the LNCaP-AI cell line increased migration and invasion (P < 0.01). DVL2 protein level was up-regulated after transfection of anti-miR-221. MiR-221 was up-regulated in CaP plasma (P < 0.01). We demonstrate a significant difference in miR-221 expression between ADPC and AIPC. MiR-221 may contribute to NE differentiation, which may be the cause for AIPC. We also suggest that miR-221 may control the migration of AIPC cells through DVL2, working as a key regulator in advanced CaP. The role of miR-221 in other target mRNA needs to be further investigated.
Similar articles
-
Gene microarray assessment of multiple genes and signal pathways involved in androgen-dependent prostate cancer becoming androgen independent.Asian Pac J Cancer Prev. 2014;15(22):9791-5. doi: 10.7314/apjcp.2014.15.22.9791. Asian Pac J Cancer Prev. 2014. PMID: 25520106
-
Interplay between steroid signalling and microRNAs: implications for hormone-dependent cancers.Endocr Relat Cancer. 2014 Oct;21(5):R409-29. doi: 10.1530/ERC-14-0208. Epub 2014 Jul 25. Endocr Relat Cancer. 2014. PMID: 25062737 Review.
-
Androgen receptor as a regulator of ZEB2 expression and its implications in epithelial-to-mesenchymal transition in prostate cancer.Endocr Relat Cancer. 2014 May 8;21(3):473-86. doi: 10.1530/ERC-13-0514. Print 2014 Jun. Endocr Relat Cancer. 2014. PMID: 24812058
-
Dishevelled-2 silencing reduces androgen-dependent prostate tumor cell proliferation and migration and expression of Wnt-3a and matrix metalloproteinases.Mol Biol Rep. 2013 Jul;40(7):4241-50. doi: 10.1007/s11033-013-2506-6. Epub 2013 May 8. Mol Biol Rep. 2013. PMID: 23652996
-
MicroRNAs in androgen-dependent PCa.Front Biosci (Landmark Ed). 2013 Jan 1;18(2):748-55. doi: 10.2741/4137. Front Biosci (Landmark Ed). 2013. PMID: 23276959 Review.
Cited by
-
Differential Effects of Somatostatin, Octreotide, and Lanreotide on Neuroendocrine Differentiation and Proliferation in Established and Primary NET Cell Lines: Possible Crosstalk with TGF-β Signaling.Int J Mol Sci. 2022 Dec 14;23(24):15868. doi: 10.3390/ijms232415868. Int J Mol Sci. 2022. PMID: 36555512 Free PMC article.
-
miR-221/222 as biomarkers and targets for therapeutic intervention on cancer and other diseases: A systematic review.Mol Ther Nucleic Acids. 2022 Feb 11;27:1191-1224. doi: 10.1016/j.omtn.2022.02.005. eCollection 2022 Mar 8. Mol Ther Nucleic Acids. 2022. PMID: 35282417 Free PMC article. Review.
-
Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer.PLoS One. 2021 Dec 31;16(12):e0262017. doi: 10.1371/journal.pone.0262017. eCollection 2021. PLoS One. 2021. PMID: 34972164 Free PMC article.
-
Regulation of Neuroendocrine-like Differentiation in Prostate Cancer by Non-Coding RNAs.Noncoding RNA. 2021 Dec 2;7(4):75. doi: 10.3390/ncrna7040075. Noncoding RNA. 2021. PMID: 34940756 Free PMC article. Review.
-
miRNAs as biomarkers for early cancer detection and their application in the development of new diagnostic tools.Biomed Eng Online. 2021 Feb 16;20(1):21. doi: 10.1186/s12938-021-00857-9. Biomed Eng Online. 2021. PMID: 33593374 Free PMC article. Review.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous