Sequence analysis of P gene of human parainfluenza type 2 virus: P and cysteine-rich proteins are translated by two mRNAs that differ by two nontemplated G residues
- PMID: 2162103
- DOI: 10.1016/0042-6822(90)90465-4
Sequence analysis of P gene of human parainfluenza type 2 virus: P and cysteine-rich proteins are translated by two mRNAs that differ by two nontemplated G residues
Abstract
We cloned and sequenced the cDNAs against genomic RNA and mRNA for phosphoprotein (P) of human parainfluenza type 2 virus (PIV-2). cDNA clone from genomic RNA was 1439 nucleotides in length excluding poly(A) and was found to have two small open reading frames encoding proteins of 233 and 249 amino acids. Two different mRNA cDNA clones were obtained; that is, one mRNA contained a smaller reading frame coding 225 amino acids, V protein, and the other mRNA contained a larger reading frame coding 395 amino acids, P protein. Both mRNAs had G cluster in coding frame. The former mRNA contained seven G residues, and two extra G residues were inserted in the latter mRNA. Ten cDNA clones from the genomic RNA were identical and were composed of seven G residues, indicating that genomes analyzed here were a homogeneous population. Therefore, V protein is encoded by faithfully copied mRNA and P protein is translated from mRNA in which two additional G residues are nontemplately inserted immediately after seven genomically encoded G residues. The V and P proteins are amino coterminal proteins and have different C termini. The C terminus of V protein is cysteine-rich and bears some resemblance to metal-binding protein of the zinc finger-type motif. P protein sequence of PIV-2 showed high homologies with SV 5 (40.4%) and mumps virus (35.5%), and a moderate homology with Newcastle disease virus (20.6%). On the other hand, very little homology was found between PIV-2 and other paramyxoviruses including Sendai virus, PIV-3, and measles virus. The cysteine-rich region in V protein was found to be highly conserved in PIV-2, SV 5, and measles virus, suggesting that V protein of paramyxoviruses plays important roles in transcription and/or replication. The predicted cysteine-rich V protein was detected in virus-infected cells using antiserum directed against an oligopeptide specific for the predicted V polypeptide.
Similar articles
-
Sequence analyses of the 3' genome end and NP gene of human parainfluenza type 2 virus: sequence variation of the gene-starting signal and the conserved 3' end.Virology. 1990 Dec;179(2):777-84. doi: 10.1016/0042-6822(90)90145-h. Virology. 1990. PMID: 2173261
-
Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise.Virology. 1990 Sep;178(1):321-6. doi: 10.1016/0042-6822(90)90413-l. Virology. 1990. PMID: 2167560
-
Two nontemplated nucleotide additions are required to generate the P mRNA of parainfluenza virus type 2 since the RNA genome encodes protein V.Virology. 1990 Jul;177(1):388-90. doi: 10.1016/0042-6822(90)90497-f. Virology. 1990. PMID: 2162111
-
The molecular biology of the porcine paramyxovirus LPMV.Vet Microbiol. 1992 Nov;33(1-4):263-73. doi: 10.1016/0378-1135(92)90054-w. Vet Microbiol. 1992. PMID: 1481359 Review.
-
Loss of V protein expression in human parainfluenza virus type 1 is not a recent event.Virus Res. 1992 Jul;24(2):137-44. doi: 10.1016/0168-1702(92)90002-q. Virus Res. 1992. PMID: 1326826 Review.
Cited by
-
Evolutionary history of cotranscriptional editing in the paramyxoviral phosphoprotein gene.Virus Evol. 2021 Mar 27;7(1):veab028. doi: 10.1093/ve/veab028. eCollection 2021 Jan. Virus Evol. 2021. PMID: 34141448 Free PMC article. Review.
-
Human Parainfluenza Virus Type 2 V Protein Modulates Iron Homeostasis.J Virol. 2021 Feb 24;95(6):e01861-20. doi: 10.1128/JVI.01861-20. Print 2021 Feb 24. J Virol. 2021. PMID: 33408172 Free PMC article.
-
Inhibition of Cavin3 Degradation by the Human Parainfluenza Virus Type 2 V Protein Is Important for Efficient Viral Growth.Front Microbiol. 2020 Apr 30;11:803. doi: 10.3389/fmicb.2020.00803. eCollection 2020. Front Microbiol. 2020. PMID: 32425917 Free PMC article.
-
Tetherin antagonism by V proteins is a common trait among the genus Rubulavirus.Med Microbiol Immunol. 2017 Aug;206(4):319-326. doi: 10.1007/s00430-017-0509-y. Epub 2017 May 2. Med Microbiol Immunol. 2017. PMID: 28466381
-
Graf1 Controls the Growth of Human Parainfluenza Virus Type 2 through Inactivation of RhoA Signaling.J Virol. 2016 Sep 29;90(20):9394-405. doi: 10.1128/JVI.01471-16. Print 2016 Oct 15. J Virol. 2016. PMID: 27512058 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Other Literature Sources