Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Dec 7;18(45):6651-6.
doi: 10.3748/wjg.v18.i45.6651.

Overexpression of lysine specific demethylase 1 predicts worse prognosis in primary hepatocellular carcinoma patients

Ze-Kun Zhao  1 , Hai-Feng Yu, Dao-Rong Wang, Ping Dong, Lei Chen, Wen-Guang Wu, Wen-Jun Ding, Ying-Bin Liu
Affiliations

Overexpression of lysine specific demethylase 1 predicts worse prognosis in primary hepatocellular carcinoma patients

Ze-Kun Zhao et al. World J Gastroenterol. .

Abstract

Aim: To investigate the clinicopathological features and prognostic value of lysine specific demethylase 1 (LSD1) in hepatocellular carcinoma (HCC).

Methods: We examined LSD1 expression in 60 paired liver cancer tissues and adjacent noncancerous tissues by quantitative real time polymerase chain reaction (qRT-PCR) and Western blotting. In addition, we analyzed LSD1 expression in 198 HCC samples by immunohistochemistry. The relationship between LSD1 expression, clinicopathological features and patient survival was investigated.

Results: Immunohistochemistry, Western blotting, and qRT-PCR consistently confirmed LSD1 overexpression in HCC tissues compared to adjacent non-neoplastic tissues (P < 0.01). Additionally, immunostaining showed more LSD1-positive cells in the higher tumor stage (T3-4) and tumor grade (G3) than in the lower tumor stage (T1-2, P < 0.001) and tumor grade (G1-2, P < 0.001), respectively. Moreover, HCC patients with high LSD1 expression had significantly lower 5-year overall survival rates (P < 0.001) and lower 5-year disease-free survival rates (P < 0.001), respectively. A Cox proportional hazards model further demonstrated that LSD1 over-expression was an independent predictor of poor prognosis for both 5-year disease-free survival [hazards ratio (HR) = 1.426, 95%CI: 0.672-2.146, P < 0.001] and 5-year overall survival (HR = 2.456, 95%CI: 1.234-3.932, P < 0.001) in HCC.

Conclusion: Our data suggest for the first time that the overexpression of LSD1 protein in HCC tissues indicates tumor progression and predicts poor prognosis.

Keywords: Hepatocellular carcinoma; Lysine specific demethylase 1; Prognosis; Tumor progression.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Lysine specific demethylase 1 expression in hepatocellular carcinoma and adjacent non-neoplastic liver tissues. A: High expression of lysine specific demethylase 1 (LSD1) in hepatocellular carcinoma (HCC) samples; B: Low expression of LSD1 in HCC samples (original magnification, 200×).
Figure 2
Figure 2
Increased lysine specific demethylase 1 protein and mRNA levels in hepatocellular carcinoma with different tumor node metastasis stages and adjacent non-neoplastic liver tissues. A: Representative Western blotting of lysine specific demethylase 1 (LSD1) protein levels in hepatocellular carcinoma (HCC) tissues (T) and adjacent non-neoplastic liver tissues (N); B: Semiquantitative Western blotting showed that the expression levels of LSD1 protein were significantly higher than those in adjacent non-neoplastic liver tissues (P < 0.01). Additionally, the expression levels of LSD1 protein increased with ascending tumor node metastasis (TNM) stages. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. P values, mean and SD were given (t test); C: Quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay showed significantly increased LSD1 mRNA levels in HCC tissues compared with adjacent non-neoplastic liver tissues (P < 0.01). Additionally, the expression levels of LSD1 mRNA were increased with ascending tumor TNM stages. GAPDH was used as the internal control. P values, mean and SD were given (Mann-Whitney test).
Figure 3
Figure 3
Kaplan-Meier survival curves for lysine specific demethylase 1 expression in hepatocellular carcinoma patients. A: The hepatocellular carcinoma patients with high lysine specific demethylase 1 (LSD1) expression showed significantly shorter disease-free survival (P < 0.001); B: Overall survival (P < 0.001) rates than those with low LSD1 expression.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Schütte K, Bornschein J, Malfertheiner P. Hepatocellular carcinoma--epidemiological trends and risk factors. Dig Dis. 2009;27:80–92. - PubMed
    1. Li D, Mallory T, Satomura S. AFP-L3: a new generation of tumor marker for hepatocellular carcinoma. Clin Chim Acta. 2001;313:15–19. - PubMed
    1. Blum HE, Spangenberg HC. Hepatocellular carcinoma: an update. Arch Iran Med. 2007;10:361–371. - PubMed
    1. Wang J, Scully K, Zhu X, Cai L, Zhang J, Prefontaine GG, Krones A, Ohgi KA, Zhu P, Garcia-Bassets I, et al. Opposing LSD1 complexes function in developmental gene activation and repression programmes. Nature. 2007;446:882–887. - PubMed
    1. Scoumanne A, Chen X. The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners. J Biol Chem. 2007;282:15471–15475. - PubMed
-