Competitive chemiluminescent enzyme immunoassay for vitamin B12 analysis in human milk
- PMID: 24491700
- DOI: 10.1016/j.foodchem.2013.12.033
Competitive chemiluminescent enzyme immunoassay for vitamin B12 analysis in human milk
Abstract
Recent discoveries of matrix interferences by haptocorrin (HC) in human milk and serum show that past analyses of vitamin B12 in samples with high HC content might have been inaccurate (Lildballe et al., 2009; Carmel & Agrawal, 2012). We evaluated two competitive enzyme-binding immunoassays for serum/plasma (IMMULITE and SimulTRAC-SNB) for B12 analysis in human milk. B12-recovery rates (United States Environmental Protection Agency, 2007) were determined to be 78.9 ± 9.1% with IMMULITE and 225 ± 108% (range 116-553%) using SimulTRAC-SNB, most likely due to the presence of excess HC. HC-interferences were not observed with the IMMULITE assay, rendering previously reported mandatory HC-removal (Lildballe et al., 2009) unnecessary. Linearity continued at low B12-concentrations (24-193 pM; r(2)>0.985). Milk B12 concentrations from Bangladeshi women (72-959 pM) were significantly lower than those from California (154-933 pM; p<0.0001) showing IMMULITE's robustness against the complex milk matrix and its ability to measure low milk B12 concentrations.
Keywords: Chemiluminescence; Cobalamin; Haptocorrin; Human milk; Radioassay; Vitamin B12.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Similar articles
-
Vitamin B12 concentrations in milk from Norwegian women during the six first months of lactation.Eur J Clin Nutr. 2020 May;74(5):749-756. doi: 10.1038/s41430-020-0567-x. Epub 2020 Jan 30. Eur J Clin Nutr. 2020. PMID: 32001810 Free PMC article.
-
Vitamin B12 and its binding proteins in milk from cow and buffalo in relation to bioavailability of B12.J Dairy Sci. 2019 Jun;102(6):4891-4905. doi: 10.3168/jds.2018-15016. Epub 2019 Mar 28. J Dairy Sci. 2019. PMID: 30928264
-
Binding of transcobalamin II by human mammary epithelial cells.Adv Exp Med Biol. 2001;501:469-77. doi: 10.1007/978-1-4615-1371-1_58. Adv Exp Med Biol. 2001. PMID: 11787717
-
Vitamin B12 levels in ewe colostrum and milk and in lamb serum.Vet Res. 1994;25(4):405-9. Vet Res. 1994. PMID: 8087148
-
Iron, zinc, folate and vitamin B12 nutritional status and milk composition of low-income Brazilian mothers.Eur J Clin Nutr. 1989 Apr;43(4):253-66. Eur J Clin Nutr. 1989. PMID: 2661218 Review.
Cited by
-
The Fast Quantification of Vitamin B12 in Milk Powder by High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry.Molecules. 2024 Apr 15;29(8):1795. doi: 10.3390/molecules29081795. Molecules. 2024. PMID: 38675615 Free PMC article.
-
Effects of vitamin B12 supplementation on oxidative stress markers and pro-inflammatory cytokines during pregnancy and postpartum among Bangladeshi mother-child pairs.BMC Nutr. 2024 Jan 3;10(1):3. doi: 10.1186/s40795-023-00785-y. BMC Nutr. 2024. PMID: 38172996 Free PMC article.
-
Mothers' Breast Milk Composition and Their Respective Infant's Gut Microbiota Differ between Five Distinct Rural and Urban Regions in Vietnam.Nutrients. 2023 Nov 16;15(22):4802. doi: 10.3390/nu15224802. Nutrients. 2023. PMID: 38004196 Free PMC article.
-
Maternal Mental Health in Late Pregnancy and Longitudinal Changes in Postpartum Serum Vitamin B-12, Homocysteine, and Milk B-12 Concentration Among Brazilian Women.Front Nutr. 2022 Jul 11;9:923569. doi: 10.3389/fnut.2022.923569. eCollection 2022. Front Nutr. 2022. PMID: 35898719 Free PMC article.
-
Chicken liver and eggshell crackers as a safe and affordable animal source food for overcoming micronutrient deficits during pregnancy and lactation in Indonesia: a double-blind, randomised placebo-controlled trial (SISTIK Growth Study).Wellcome Open Res. 2022 Jun 9;7:167. doi: 10.12688/wellcomeopenres.17879.1. eCollection 2022. Wellcome Open Res. 2022. PMID: 35865219 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
