A draft map of the human proteome
- PMID: 24870542
- PMCID: PMC4403737
- DOI: 10.1038/nature13302
A draft map of the human proteome
Abstract
The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.
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Comment in
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Analyzing the first drafts of the human proteome.J Proteome Res. 2014 Aug 1;13(8):3854-5. doi: 10.1021/pr500572z. Epub 2014 Jul 16. J Proteome Res. 2014. PMID: 25014353 Free PMC article.
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The human proteome takes the spotlight.Nat Methods. 2014 Jul;11(7):709. doi: 10.1038/nmeth.3017. Nat Methods. 2014. PMID: 25110780 No abstract available.
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Pharmaceutical relevant cytokine receptors: lessons from the first drafts of the human proteome.J Proteome Res. 2015 Feb 6;14(2):1330-2. doi: 10.1021/pr500875b. Epub 2014 Dec 8. J Proteome Res. 2015. PMID: 25437751
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The potential clinical impact of the release of two drafts of the human proteome.Expert Rev Proteomics. 2015;12(6):579-93. doi: 10.1586/14789450.2015.1103186. Epub 2015 Oct 23. Expert Rev Proteomics. 2015. PMID: 26496066 Free PMC article.
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