Human miR-5193 Triggers Gene Silencing in Multiple Genotypes of Hepatitis B Virus
- PMID: 26456535
- DOI: 10.2174/2211536604666150819195743
Human miR-5193 Triggers Gene Silencing in Multiple Genotypes of Hepatitis B Virus
Abstract
Hepatitis B virus (HBV) infection can lead to various disease states including asymptomatic, acute hepatitis, chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC), which remain a major health problem worldwide. Previous studies demonstrated that microRNA (miRNAs) plays an important role in viral replication. This study aimed to predict and evaluate human miRNAs targeting multiple genotypes of HBV. Candidate human miRNAs were analyzed by data obtained from miRBase and RNAhybrid. Then miRNAs were selected based on hybridization patterns and minimum free energy (MFE). The silencing effect of miRNA was evaluated by real-time PCR, the luciferase reporter assay and the ELISA assay. Five human miRNAs including miR- 142-5p, miR-384, miR-500b, miR-4731-5p and miR-5193 were found to target several HBV genotypes. Interestingly, miR-5193 was found to be the most potent miRNA that could target against all HBV transcripts in almost all HBV genotypes with a highly stable hybridization pattern (5' canonical with MFE lower than -35 kcal/mol). Moreover, miR-5193 caused significant silencing in luciferase activity (53% reduction), luciferase transcript (60% reduction) and HBV surface antigen (HBsAg) production (20-40% reduction depending on genotypes). Therefore, miR-5193 might be useful and have a vital role for inhibition of HBV replication in the future.
Similar articles
-
MiR-185-5p suppresses HBV gene expression by targeting ELK1 in hepatoma carcinoma cells.Life Sci. 2018 Nov 15;213:9-17. doi: 10.1016/j.lfs.2018.10.016. Epub 2018 Oct 9. Life Sci. 2018. PMID: 30308183
-
miR-200c targets nuclear factor IA to suppress HBV replication and gene expression via repressing HBV Enhancer I activity.Biomed Pharmacother. 2018 Mar;99:774-780. doi: 10.1016/j.biopha.2018.01.141. Epub 2018 Feb 20. Biomed Pharmacother. 2018. PMID: 29710475
-
Hepatitis B virus and microRNAs: Complex interactions affecting hepatitis B virus replication and hepatitis B virus-associated diseases.World J Gastroenterol. 2015 Jun 28;21(24):7375-99. doi: 10.3748/wjg.v21.i24.7375. World J Gastroenterol. 2015. PMID: 26139985 Free PMC article. Review.
-
Role of microRNAs in hepatitis B virus replication and pathogenesis.Biochim Biophys Acta. 2011 Nov-Dec;1809(11-12):678-85. doi: 10.1016/j.bbagrm.2011.04.008. Epub 2011 May 4. Biochim Biophys Acta. 2011. PMID: 21565290 Review.
-
Suppression of hepatitis B virus replication by microRNA-199a-3p and microRNA-210.Antiviral Res. 2010 Nov;88(2):169-75. doi: 10.1016/j.antiviral.2010.08.008. Epub 2010 Aug 20. Antiviral Res. 2010. PMID: 20728471
Cited by
-
Three genes expressed in relation to lipid metabolism considered as potential biomarkers for the diagnosis and treatment of diabetic peripheral neuropathy.Sci Rep. 2023 May 29;13(1):8679. doi: 10.1038/s41598-023-35908-9. Sci Rep. 2023. PMID: 37248406 Free PMC article.
-
Circular RNA Eps15-homology domain-containing protein 2 induce resistance of renal cell carcinoma to sunitinib via microRNA-4731-5p/ABCF2 axis.Bioengineered. 2022 Apr;13(4):9729-9740. doi: 10.1080/21655979.2022.2059960. Bioengineered. 2022. PMID: 35412955 Free PMC article.
-
Potential Molecular Mechanisms and Remdesivir Treatment for Acute Respiratory Syndrome Corona Virus 2 Infection/COVID 19 Through RNA Sequencing and Bioinformatics Analysis.Bioinform Biol Insights. 2021 Dec 23;15:11779322211067365. doi: 10.1177/11779322211067365. eCollection 2021. Bioinform Biol Insights. 2021. PMID: 34992355 Free PMC article.
-
DUOX2 As a Potential Prognostic Marker which Promotes Cell Motility and Proliferation in Pancreatic Cancer.Biomed Res Int. 2021 Mar 2;2021:6530298. doi: 10.1155/2021/6530298. eCollection 2021. Biomed Res Int. 2021. PMID: 33748270 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical