Magnesium and calcium-enriched deep-sea water promotes mitochondrial biogenesis by AMPK-activated signals pathway in 3T3-L1 preadipocytes
- PMID: 27434863
- DOI: 10.1016/j.biopha.2016.07.009
Magnesium and calcium-enriched deep-sea water promotes mitochondrial biogenesis by AMPK-activated signals pathway in 3T3-L1 preadipocytes
Abstract
Recent studies showed that deficiencies of essential minerals including Mg, Ca, and K, and trace minerals including Se, Zn, and V, have implications for the development, prevention, and treatment of several chronic diseases including obesity and type 2 diabetes. Our previous studies revealed that balanced deep-sea water (BDSW), which is composed of desalinated water enriched with Mg and Ca, has potential as a treatment for diabetes and obesity. In this study, to determine whether BDSW regulates mitochondrial biogenesis and function, we investigated its effects on mitochondrial DNA (mtDNA) content, mitochondrial enzyme activity, expression of key transcription factors and mitochondria-specific genes, phosphorylation of signaling molecules associated with mitochondrial biogenesis, and mitochondrial function in 3T3-L1 preadipocytes. BDSW increased mitochondrial biogenesis in a dose-dependent manner. Quantitative real-time PCR revealed that BDSW enhances expression of PGC1-α, NRF1, and TFAM genes. Upregulation of these genes was supported by increased mitochondria staining, CytC oxidase activity, and AMPK phosphorylation. The stimulatory effect of BDSW on mitochondrial biogenesis and function suggests a novel mechanism for BDSW-induced anti-diabetic and anti-obesity action.
Keywords: AMPK; Calcium; Magnesium; Mineral deficiency; Mitochondrial biogenesis.
Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Similar articles
-
Salicylates promote mitochondrial biogenesis by regulating the expression of PGC-1α in murine 3T3-L1 pre-adipocytes.Biochem Biophys Res Commun. 2017 Sep 16;491(2):436-441. doi: 10.1016/j.bbrc.2017.07.074. Epub 2017 Jul 13. Biochem Biophys Res Commun. 2017. PMID: 28712868
-
Translational regulation of mitochondrial biogenesis.Biochem Soc Trans. 2016 Dec 15;44(6):1717-1724. doi: 10.1042/BST20160071C. Biochem Soc Trans. 2016. PMID: 27913682 Review.
-
Regulation of mitochondrial biogenesis through TFAM-mitochondrial DNA interactions: Useful insights from aging and calorie restriction studies.Mitochondrion. 2015 Nov;25:67-75. doi: 10.1016/j.mito.2015.10.001. Epub 2015 Oct 3. Mitochondrion. 2015. PMID: 26437364 Review.
-
OM2, a Novel Oligomannuronate-Chromium(III) Complex, Promotes Mitochondrial Biogenesis and Lipid Metabolism in 3T3-L1 Adipocytes via the AMPK-PGC1α Pathway.PLoS One. 2015 Jul 15;10(7):e0131930. doi: 10.1371/journal.pone.0131930. eCollection 2015. PLoS One. 2015. PMID: 26176781 Free PMC article.
-
Stimulatory Effects of Balanced Deep Sea Water on Mitochondrial Biogenesis and Function.PLoS One. 2015 Jun 12;10(6):e0129972. doi: 10.1371/journal.pone.0129972. eCollection 2015. PLoS One. 2015. PMID: 26068191 Free PMC article.
Cited by
-
Marine natural products and human immunity: novel biomedical resources for anti-infection of SARS-CoV-2 and related cardiovascular disease.Nat Prod Bioprospect. 2024 Jan 29;14(1):12. doi: 10.1007/s13659-024-00432-4. Nat Prod Bioprospect. 2024. PMID: 38282092 Free PMC article. Review.
-
Magnesium Supplementation Affects the Expression of Sirtuin1, Tumor Protein P53 and Endothelial Nitric Oxide Synthase Genes in Patients with Atherosclerosis: A Double-Blind, Randomized, Placebo-Controlled Trial.Indian J Clin Biochem. 2023 Jan;38(1):59-66. doi: 10.1007/s12291-022-01032-0. Epub 2022 Apr 9. Indian J Clin Biochem. 2023. PMID: 36684501 Free PMC article.
-
Physiological Benefits and Performance of Sea Water Ingestion for Athletes in Endurance Events: A Systematic Review.Nutrients. 2022 Nov 2;14(21):4609. doi: 10.3390/nu14214609. Nutrients. 2022. PMID: 36364871 Free PMC article. Review.
-
Seawater Hydration Modulates IL-6 and Apelin Production during Triathlon Events: A Crossover Randomized Study.Int J Environ Res Public Health. 2022 Aug 4;19(15):9581. doi: 10.3390/ijerph19159581. Int J Environ Res Public Health. 2022. PMID: 35954937 Free PMC article. Clinical Trial.
-
Rutaecarpine Promotes Adipose Thermogenesis and Protects against HFD-Induced Obesity via AMPK/PGC-1α Pathway.Pharmaceuticals (Basel). 2022 Apr 13;15(4):469. doi: 10.3390/ph15040469. Pharmaceuticals (Basel). 2022. PMID: 35455466 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources