Examination of in vivo mutagenicity of sodium arsenite and dimethylarsinic acid in gpt delta rats
- PMID: 28007167
- DOI: 10.1016/j.jes.2016.07.005
Examination of in vivo mutagenicity of sodium arsenite and dimethylarsinic acid in gpt delta rats
Abstract
Arsenic is a well-known human bladder and liver carcinogen, but its exact mechanism of carcinogenicity is not fully understood. Dimethylarsinic acid (DMAV) is a major urinary metabolite of sodium arsenite (iAsIII) and induces urinary bladder cancers in rats. DMAV and iAsIII are negative in in vitro mutagenicity tests. However, their in vivo mutagenicities have not been determined. The purpose of present study is to evaluate the in vivo mutagenicities of DMAV and iAsIII in rat urinary bladder epithelium and liver using gpt delta F344 rats. Ten-week old male gpt delta F344 rats were randomized into 3 groups and administered 0, 92mg/L DMAV, or 87mg/L iAsIII (each 50mg/L As) for 13weeks in the drinking water. In the mutation assay, point mutations are detected in the gpt gene by 6-thioguanine selection (gpt assay) and deletion mutations are identified in the red/gam genes by Spi- selection (Spi- assay). Results of the gpt and Spi- assays showed that DMAV and iAsIII had no effects on the mutant frequencies or mutation spectrum in urinary bladder epithelium or liver. These findings indicate that DMAV and iAsIII are not mutagenic in urinary bladder epithelium or liver in rats.
Keywords: Dimethylarsinic acid; In vivo mutagenicity; Sodium arsenite; Urinary bladder epithelium; gpt delta rat.
Copyright © 2016. Published by Elsevier B.V.
Similar articles
-
Effects of co-administration of dietary sodium arsenite and an NADPH oxidase inhibitor on the rat bladder epithelium.Toxicology. 2009 Jun 30;261(1-2):41-6. doi: 10.1016/j.tox.2009.04.042. Epub 2009 May 3. Toxicology. 2009. PMID: 19397947
-
Possible role of dimethylarsinous acid in dimethylarsinic acid-induced urothelial toxicity and regeneration in the rat.Chem Res Toxicol. 2002 Sep;15(9):1150-7. doi: 10.1021/tx020026z. Chem Res Toxicol. 2002. PMID: 12230408
-
In vivo mutagenicity of arsenite in the livers of gpt delta transgenic mice.Mutat Res Genet Toxicol Environ Mutagen. 2014 Jan 15;760:42-7. doi: 10.1016/j.mrgentox.2013.12.001. Epub 2013 Dec 10. Mutat Res Genet Toxicol Environ Mutagen. 2014. PMID: 24333349
-
A concise review of the toxicity and carcinogenicity of dimethylarsinic acid.Toxicology. 2001 Mar 7;160(1-3):227-36. doi: 10.1016/s0300-483x(00)00458-3. Toxicology. 2001. PMID: 11246143 Review.
-
Possible carcinogenic potential of dimethylarsinic acid as assessed in rat in vivo models: a review.Mutat Res. 1997 Jun;386(3):353-61. doi: 10.1016/s1383-5742(97)00017-3. Mutat Res. 1997. PMID: 9219572 Review.
Cited by
-
Update of the risk assessment of inorganic arsenic in food.EFSA J. 2024 Jan 18;22(1):e8488. doi: 10.2903/j.efsa.2024.8488. eCollection 2024 Jan. EFSA J. 2024. PMID: 38239496 Free PMC article.
-
DNA Methylation Aberrations in Dimethylarsinic Acid-Induced Bladder Carcinogenesis.Cancers (Basel). 2023 Nov 3;15(21):5274. doi: 10.3390/cancers15215274. Cancers (Basel). 2023. PMID: 37958445 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials