Curcumin inhibits angiotensin II-induced inflammation and proliferation of rat vascular smooth muscle cells by elevating PPAR-γ activity and reducing oxidative stress
- PMID: 28339005
- DOI: 10.3892/ijmm.2017.2924
Curcumin inhibits angiotensin II-induced inflammation and proliferation of rat vascular smooth muscle cells by elevating PPAR-γ activity and reducing oxidative stress
Abstract
Angiotensin II (AngII)-induced production of inflammatory factors and proliferation in vascular smooth muscle cells (VSMCs) play an important role in the progression of atherosclerotic plaques. Growing evidence has demonstrated that activation of peroxisome proliferator-activated receptor-γ (PPAR-γ) effectively attenuates AngII-induced inflammation and intercellular reactive oxygen species (iROS) production. Curcumin (Cur) inhibits inflammatory responses by enhancing PPAR-γ activity and reducing oxidative stress in various tissues. The aim of the present study was to ascertain whether Cur inhibits AngII-induced inflammation and proliferation, and its underlying molecular mechanism, in VSMCs. Enzyme-linked immunosorbent assay (ELISA) and real-time PCR were used to measure the protein and mRNA expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Nitric oxide (NO) production was measured by Griess reaction. Western blot analysis and a DNA-binding assay were used to measure PPAR-γ activity. iROS production was measured using the DCFH-DA method. In rat VSMCs, Cur attenuated AngII‑induced expression of IL-6 and TNF-α mRNA and protein in a concentration-dependent manner, inhibited NO production by suppressing inducible NO synthase (iNOS) activity, and suppressed proliferation of VSMCs. This was accompanied by increased PPAR-γ expression and activation in Cur-pretreated VSMCs. GW9662, a PPAR-γ antagonist, reversed the anti-inflammatory effect of Cur. Moreover, Cur attenuated AngII-induced oxidative stress by downregulating the expression of p47phox, which is a key subunit of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. In conclusion, Cur inhibited the expression of IL-6 and TNF-α, decreased the production of NO, and suppressed the proliferation of VSMCs, by elevating PPAR-γ activity and suppressing oxidative stress, leading to attenuated AngII-induced inflammatory responses in VSMCs.
Similar articles
-
NF-κB pathway as a molecular target for curcumin in diabetes mellitus treatment: Focusing on oxidative stress and inflammation.Cell Biochem Funct. 2024 Jun;42(4):e4030. doi: 10.1002/cbf.4030. Cell Biochem Funct. 2024. PMID: 38720663 Review.
-
Metformin inhibits LPS-induced inflammatory response in VSMCs by regulating TLR4 and PPAR-γ.Eur Rev Med Pharmacol Sci. 2019 Jun;23(11):4988-4995. doi: 10.26355/eurrev_201906_18090. Eur Rev Med Pharmacol Sci. 2019. PMID: 31210335
-
Thymoquinone Inhibits Angiotensin II-Induced Proliferation and Migration of Vascular Smooth Muscle Cells Through the AMPK/PPARγ/PGC-1α Pathway.DNA Cell Biol. 2016 Aug;35(8):426-33. doi: 10.1089/dna.2016.3262. Epub 2016 Apr 11. DNA Cell Biol. 2016. PMID: 27064837
-
Demethoxycurcumin, a natural derivative of curcumin attenuates LPS-induced pro-inflammatory responses through down-regulation of intracellular ROS-related MAPK/NF-kappaB signaling pathways in N9 microglia induced by lipopolysaccharide.Int Immunopharmacol. 2010 Mar;10(3):331-8. doi: 10.1016/j.intimp.2009.12.004. Epub 2009 Dec 16. Int Immunopharmacol. 2010. PMID: 20018257
-
Regulation of smooth muscle by inducible nitric oxide synthase and NADPH oxidase in vascular proliferative diseases.Free Radic Biol Med. 2008 Apr 1;44(7):1232-45. doi: 10.1016/j.freeradbiomed.2007.12.025. Epub 2008 Jan 22. Free Radic Biol Med. 2008. PMID: 18211830 Free PMC article. Review.
Cited by
-
Canagliflozin alleviates pulmonary hypertension by activating PPARγ and inhibiting its S225 phosphorylation.Acta Pharmacol Sin. 2024 May 8. doi: 10.1038/s41401-024-01286-9. Online ahead of print. Acta Pharmacol Sin. 2024. PMID: 38719955
-
Potential of Curcumin in the Management of Skin Diseases.Int J Mol Sci. 2024 Mar 23;25(7):3617. doi: 10.3390/ijms25073617. Int J Mol Sci. 2024. PMID: 38612433 Free PMC article. Review.
-
Potential of Plant-Derived Compounds in Preventing and Reversing Organ Fibrosis and the Underlying Mechanisms.Cells. 2024 Feb 28;13(5):421. doi: 10.3390/cells13050421. Cells. 2024. PMID: 38474385 Free PMC article. Review.
-
Hexahydrocurcumin mitigates angiotensin II-induced proliferation, migration, and inflammation in vascular smooth muscle cells.EXCLI J. 2023 Jun 5;22:466-481. doi: 10.17179/excli2023-6124. eCollection 2023. EXCLI J. 2023. PMID: 37534221 Free PMC article.
-
Superoxide dismutase: a key target for the neuroprotective effects of curcumin.Mol Cell Biochem. 2024 Mar;479(3):693-705. doi: 10.1007/s11010-023-04757-5. Epub 2023 May 11. Mol Cell Biochem. 2024. PMID: 37166541 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources