Effects of tris(hydroxymethyl)aminomethane on biosynthesis and release of insulin in the pancreatic Langerhans islets
- PMID: 2873007
- DOI: 10.1016/s0168-8227(85)80031-0
Effects of tris(hydroxymethyl)aminomethane on biosynthesis and release of insulin in the pancreatic Langerhans islets
Abstract
Tris(hydroxymethyl)aminomethane (Tris) has been shown to inhibit selectively the Golgi apparatus and Golgi-endoplasmic reticulum-lysosomal system (GERL system) of several kinds of cells including pancreatic B cells. This study was designed to assess the effect of Tris on insulin, glucagon and somatostatin release and insulin synthesis in pancreatic B cells by using isolated rat pancreatic islets. Tris suppressed glucose-induced insulin release, whereas it did not affect the glucagon and somatostatin release. Furthermore, the incorporation of [3H]leucine into the insulin fraction was suppressed by 10 mM Tris, but the sum of the radioactivity of both proinsulin and insulin fraction were not influenced. The present study suggests that the Golgi apparatus and GERL system may play a role in insulin secretion and biosynthesis in pancreatic B cells.
Similar articles
-
Biosynthesis of insulin and glucagon.Methods Cell Biol. 1981;23:73-88. doi: 10.1016/s0091-679x(08)61492-2. Methods Cell Biol. 1981. PMID: 7035811 Review. No abstract available.
-
Tris (hydroxymethyl) aminomethane (THAM) induced stimulation of insulin release by islets of Langerhans previously isolated from rat pancreas.Diabetes. 1970 Aug;19(8):559-62. doi: 10.2337/diab.19.8.559. Diabetes. 1970. PMID: 4915446 No abstract available.
-
The biosynthesis and secretion of insulin.Clin Endocrinol Metab. 1972 Nov;1(3):601-22. doi: 10.1016/s0300-595x(72)80036-7. Clin Endocrinol Metab. 1972. PMID: 4359227 Review. No abstract available.
-
Tris(hydroxymethyl)aminomethane inhibits the synthesis and processing of proinsulin in isolated rat pancreatic islets without affecting release of insulin stores.Diabetes. 1986 Apr;35(4):433-9. doi: 10.2337/diab.35.4.433. Diabetes. 1986. PMID: 3514324
-
Biosynthesis of proinsulin and insulin in newborn rat pancreas. Interaction of glucose, cyclic AMP, somatostatin, and sulfonylureas on the (3H) leucine incorporation into immunoreactive insulin.J Clin Invest. 1976 Jan;57(1):230-43. doi: 10.1172/JCI108264. J Clin Invest. 1976. PMID: 173741 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical