Biophysical evidence for differential gallated green tea catechins binding to membrane type-1 matrix metalloproteinase and its interactors
- PMID: 29407769
- DOI: 10.1016/j.bpc.2018.01.002
Biophysical evidence for differential gallated green tea catechins binding to membrane type-1 matrix metalloproteinase and its interactors
Abstract
Membrane type-1 matrix metalloproteinase (MT1-MMP) is a transmembrane MMP which triggers intracellular signaling and regulates extracellular matrix proteolysis, two functions that are critical for tumor-associated angiogenesis and inflammation. While green tea catechins, particularly epigallocatechin gallate (EGCG), are considered very effective in preventing MT1-MMP-mediated functions, lack of structure-function studies and evidence regarding their direct interaction with MT1-MMP-mediated biological activities remain. Here, we assessed the impact in both cellular and biophysical assays of four ungallated catechins along with their gallated counterparts on MT1-MMP-mediated functions and molecular binding partners. Concanavalin-A (ConA) was used to trigger MT1-MMP-mediated proMMP-2 activation, expression of MT1-MMP and of endoplasmic reticulum stress biomarker GRP78 in U87 glioblastoma cells. We found that ConA-mediated MT1-MMP induction was inhibited by EGCG and catechin gallate (CG), that GRP78 induction was inhibited by EGCG, CG, and gallocatechin gallate (GCG), whereas proMMP-2 activation was inhibited by EGCG and GCG. Surface plasmon resonance was used to assess direct interaction between catechins and MT1-MMP interactors. We found that gallated catechins interacted better than their ungallated analogs with MT1-MMP as well as with MT1-MMP binding partners MMP-2, TIMP-2, MTCBP-1 and LRP1-clusterIV. Overall, current structure-function evidence supports a role for the galloyl moiety in both direct and indirect interactions of green tea catechins with MT1-MMP-mediated oncogenic processes.
Keywords: Catechins; Green tea; MT1-MMP; Polyphenols; Surface plasmon resonance.
Copyright © 2018 Elsevier B.V. All rights reserved.
Similar articles
-
Inhibition of pro-/active MMP-2 by green tea catechins and prediction of their interaction by molecular docking studies.Mol Cell Biochem. 2017 Mar;427(1-2):111-122. doi: 10.1007/s11010-016-2903-y. Epub 2016 Dec 24. Mol Cell Biochem. 2017. PMID: 28013477
-
Inhibitory Effects of Eight Green Tea Catechins on Cytochrome P450 1A2, 2C9, 2D6, and 3A4 Activities.J Pharm Pharm Sci. 2016 Apr-Jun;19(2):188-97. doi: 10.18433/J3MS5C. J Pharm Pharm Sci. 2016. PMID: 27518169 Review.
-
Multifunctional effects of green tea catechins on prevention of the metabolic syndrome.Asia Pac J Clin Nutr. 2008;17 Suppl 1:273-4. Asia Pac J Clin Nutr. 2008. PMID: 18296354 Review.
-
Green tea polyphenol (-)-epigallocatechin 3-gallate inhibits MMP-2 secretion and MT1-MMP-driven migration in glioblastoma cells.Biochim Biophys Acta. 2002 Jan 30;1542(1-3):209-20. doi: 10.1016/s0167-4889(01)00187-2. Biochim Biophys Acta. 2002. PMID: 11853893
-
Matrix metalloproteinase inhibition by green tea catechins.Biochim Biophys Acta. 2000 Mar 16;1478(1):51-60. doi: 10.1016/s0167-4838(00)00009-1. Biochim Biophys Acta. 2000. PMID: 10719174
Cited by
-
Gallation and B-Ring Dihydroxylation Increase Green Tea Catechin Residence Time in Plasma by Differentially Affecting Tissue-Specific Trafficking: Compartmental Model of Catechin Kinetics in Healthy Adults.Nutrients. 2023 Sep 17;15(18):4021. doi: 10.3390/nu15184021. Nutrients. 2023. PMID: 37764804 Free PMC article.
-
Anti-Inflammatory Effects of Dietary Polyphenols through Inhibitory Activity against Metalloproteinases.Molecules. 2023 Jul 15;28(14):5426. doi: 10.3390/molecules28145426. Molecules. 2023. PMID: 37513300 Free PMC article. Review.
-
Release and MMP-9 Inhibition Assessment of Dental Adhesive Modified with EGCG-Encapsulated Halloysite Nanotubes.Nanomaterials (Basel). 2023 Mar 9;13(6):999. doi: 10.3390/nano13060999. Nanomaterials (Basel). 2023. PMID: 36985892 Free PMC article.
-
Anticancer Mechanism of Flavonoids on High-Grade Adult-Type Diffuse Gliomas.Nutrients. 2023 Feb 4;15(4):797. doi: 10.3390/nu15040797. Nutrients. 2023. PMID: 36839156 Free PMC article. Review.
-
Effects of Epigallocatechin-3-Gallate on Matrix Metalloproteinases in Terms of Its Anticancer Activity.Molecules. 2023 Jan 5;28(2):525. doi: 10.3390/molecules28020525. Molecules. 2023. PMID: 36677584 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous