Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988:(19):203-6.

Selenocysteine on glutathione peroxidase may be converted from phosphoserine on the apo-enzyme synthesized with an opal suppressor phosphoseryl-tRNA

T Mizutani  1 , T Hitaka
Affiliations
  • PMID: 2976157

Selenocysteine on glutathione peroxidase may be converted from phosphoserine on the apo-enzyme synthesized with an opal suppressor phosphoseryl-tRNA

T Mizutani et al. Nucleic Acids Symp Ser. 1988.

Abstract

There are two possible mechanisms (co- or post-translational) for incorporation of Se into glutathione peroxidase in which selenocysteine presents at the active site of the enzyme and corresponds to UGA on the mRNA. We studied the above mechanisms using opal suppressor tRNA in mammals. Opal suppressor tRNA did not accept any selenocysteine and phosphoseryl-tRNA did not change to selenocysteyl-tRNA. Meanwhile, phosphoprotein changed to a protein containing selenocysteine by the incubation with H2Se and some enzymes. From these results, we propose that phosphoserine on glutathione peroxidase (apo-enzyme), which is synthesized with phosphoseryl-tRNA, is converted to selenocysteine in the mature enzyme, by a posttranslational mechanism. Opal suppressor tRNA may play a role to synthesize the apo-enzyme of glutathione peroxidase.

PubMed Disclaimer

Similar articles

See all similar articles

LinkOut - more resources

-