The Impact of Single-Cell Genomics on Adipose Tissue Research

doi:10.3390/ijms21134773

Review

. 2020 Jul 5;21(13):4773.

doi: 10.3390/ijms21134773.

The Impact of Single-Cell Genomics on Adipose Tissue Research

Alana Deutsch¹, Daorong Feng^{1

2}, Jeffrey E Pessin^{1

2

3}, Kosaku Shinoda^{1

2

3}

Affiliations

¹ Departments of Medicine, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.
² Departments of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.
³ Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.

PMID: 32635651
PMCID: PMC7369959
DOI: 10.3390/ijms21134773

Review

The Impact of Single-Cell Genomics on Adipose Tissue Research

Alana Deutsch et al. Int J Mol Sci. 2020.

. 2020 Jul 5;21(13):4773.

doi: 10.3390/ijms21134773.

Authors

Alana Deutsch¹, Daorong Feng^{1

2}, Jeffrey E Pessin^{1

2

3}, Kosaku Shinoda^{1

2

3}

Affiliations

¹ Departments of Medicine, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.
² Departments of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.
³ Fleischer Institute of Diabetes and Metabolism, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA.

PMID: 32635651
PMCID: PMC7369959
DOI: 10.3390/ijms21134773

Abstract

Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect tissue heterogeneity through the transcriptional characterization of individual cells, is responsible for facilitating critical advancements in this area. The unique investigative capabilities achieved by the combination of nanotechnology, molecular biology, and informatics are expanding our understanding of adipose tissue's composition and compartmentalized functional specialization, which underlie physiologic and pathogenic states, including adaptive thermogenesis, adipose tissue aging, and obesity. In this review, we will summarize the use of scRNA-seq and single-nuclei RNA-seq (snRNA-seq) in adipocyte biology and their applications to obesity and diabetes research in the hopes of increasing awareness of the capabilities of this technology and acting as a catalyst for its expanded use in further investigation.

Keywords: adipocyte; adipose; genomics; molecular metabolism; obesity; transcriptome.

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Conflict of interest statement

The authors declare no competing interest.

Figures

**Figure 1**
The workflow of single-cell-level RNA-sequencing of Brown Adipose Tissue (BAT) and White Adipose Tissue (WAT).

**Figure 2**
Single-cell genomics uncovers cellular heterogeneity in the adipose tissue and phenotypic changes during obesity.

**Figure 3**
Single-cell genomics identifies a subset of adipocyte precursor cells (APCs) and molecular regulators critical for the beiging of White Adipose Tissue (WAT).

See this image and copyright information in PMC

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References

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1. Gao H., Volat F., Sandhow L., Galitzky J., Nguyen T., Esteve D., Åström G., Mejhert N., LeDoux S., Thalamas C., et al. CD36 Is a Marker of Human Adipocyte Progenitors with Pronounced Adipogenic and Triglyceride Accumulation Potential. STEM CELLS. 2017;35:1799–1814. doi: 10.1002/stem.2635. - DOI - PubMed

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[1] Hedlund E., Deng Q. Single-cell RNA sequencing: Technical advancements and biological applications. Mol. Asp. Med. 2018;59:36–46. doi: 10.1016/j.mam.2017.07.003. - DOI - PubMed

[2] Hedlund E., Deng Q. Single-cell RNA sequencing: Technical advancements and biological applications. Mol. Asp. Med. 2018;59:36–46. doi: 10.1016/j.mam.2017.07.003. - DOI - PubMed

[3] Rondini E.A., Granneman J.G. Single cell approaches to address adipose tissue stromal cell heterogeneity. Biochem. J. 2020;477:583–600. doi: 10.1042/BCJ20190467. - DOI - PubMed

[4] Rondini E.A., Granneman J.G. Single cell approaches to address adipose tissue stromal cell heterogeneity. Biochem. J. 2020;477:583–600. doi: 10.1042/BCJ20190467. - DOI - PubMed

[5] Shinoda K., Luijten I.H.N., Hasegawa Y., Hong H., Sonne S.B., Kim M., Xue R., Chondronikola M., Cypess A.M., Tseng Y.-H., et al. Genetic and functional characterization of clonally derived adult human brown adipocytes. Nat. Med. 2015;21:389–394. doi: 10.1038/nm.3819. - DOI - PMC - PubMed

[6] Shinoda K., Luijten I.H.N., Hasegawa Y., Hong H., Sonne S.B., Kim M., Xue R., Chondronikola M., Cypess A.M., Tseng Y.-H., et al. Genetic and functional characterization of clonally derived adult human brown adipocytes. Nat. Med. 2015;21:389–394. doi: 10.1038/nm.3819. - DOI - PMC - PubMed

[7] Xue R., Lynes M.D., Dreyfuss J.M., Shamsi F., Schulz T., Zhang H., Huang T.L., Townsend K.L., Li Y., Takahashi H., et al. Clonal analyses and gene profiling identify genetic biomarkers of the thermogenic potential of human brown and white preadipocytes. Nat. Med. 2015;21:760–768. doi: 10.1038/nm.3881. - DOI - PMC - PubMed

[8] Xue R., Lynes M.D., Dreyfuss J.M., Shamsi F., Schulz T., Zhang H., Huang T.L., Townsend K.L., Li Y., Takahashi H., et al. Clonal analyses and gene profiling identify genetic biomarkers of the thermogenic potential of human brown and white preadipocytes. Nat. Med. 2015;21:760–768. doi: 10.1038/nm.3881. - DOI - PMC - PubMed

[9] Gao H., Volat F., Sandhow L., Galitzky J., Nguyen T., Esteve D., Åström G., Mejhert N., LeDoux S., Thalamas C., et al. CD36 Is a Marker of Human Adipocyte Progenitors with Pronounced Adipogenic and Triglyceride Accumulation Potential. STEM CELLS. 2017;35:1799–1814. doi: 10.1002/stem.2635. - DOI - PubMed

[10] Gao H., Volat F., Sandhow L., Galitzky J., Nguyen T., Esteve D., Åström G., Mejhert N., LeDoux S., Thalamas C., et al. CD36 Is a Marker of Human Adipocyte Progenitors with Pronounced Adipogenic and Triglyceride Accumulation Potential. STEM CELLS. 2017;35:1799–1814. doi: 10.1002/stem.2635. - DOI - PubMed

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The Impact of Single-Cell Genomics on Adipose Tissue Research

Affiliations

The Impact of Single-Cell Genomics on Adipose Tissue Research

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

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References

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Abstract

Conflict of interest statement

Figures

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Cited by

References

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