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. 2021 Nov 24;13(12):4212.
doi: 10.3390/nu13124212.

Trans-ε-Viniferin Encapsulation in Multi-Lamellar Liposomes: Consequences on Pharmacokinetic Parameters, Biodistribution and Glucuronide Formation in Rats

Pauline Beaumont  1 , Chrystel Faure  2 , Arnaud Courtois  1   3 , Michael Jourdes  1 , Axel Marchal  1 , Pierre-Louis Teissedre  1 , Tristan Richard  1 , Claude Atgié  2 , Stéphanie Krisa  1
Affiliations

Trans-ε-Viniferin Encapsulation in Multi-Lamellar Liposomes: Consequences on Pharmacokinetic Parameters, Biodistribution and Glucuronide Formation in Rats

Pauline Beaumont et al. Nutrients. .

Abstract

Trans-ε-viniferin (εVin) is a resveratrol dimer exhibiting promising biological activities for human health. Its bioavailability being low, the development of encapsulation methods would be used to overcome this issue. The aim of this study was to measure the consequences of the encapsulation of εVin in multilamellar liposomes on its pharmacokinetic parameters, metabolism and tissue distribution in rats. After oral administration of εVin (20 mg/kg body weight), either as free or encapsulated forms, plasmas were sequentially collected (from 0 to 4 h) as well as liver, kidneys and adipose tissues (4 h after administration) and analyzed by LC-HRMS. The glucuronide metabolites (εVG) were also produced by hemisynthesis for their quantification in plasma and tissues. The encapsulation process did not significantly modify the pharmacokinetic parameters of εVin itself. However, a significant increase of the T1/2 was noticed for εVG after administration of the encapsulated form as compared to the free form. An accumulation of εVin and εVG in adipose tissues was noticed, and interestingly a significant increase of the latter in the mesenteric one after administration of the encapsulated form was highlighted. Since adipose tissues could represent storage depots, and encapsulation allows for prolonging the exposure time of glucuronide metabolites in the organism, this could be of interest to promote their potential biological activities.

Keywords: Trans-ε-viniferin; biodistribution; liposome encapsulation; pharmacokinetic; resveratrol dimer.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structure of the 4 isomers of εVin. (a) basic structure; (b) structure of V2G; (c) location of glucuronide group (gluc) depending on the isomer.
Figure 2
Figure 2
Kinetic profiles of εVin (a) and εVG (b) after oral administration of 20 mg/kg bw of free (white triangles) or encapsulated (black circles) εVin. Data are expressed in pmol/mL of plasma + standard deviation (SD). Lambda z and AUC0–∞ are expressed in min−1 and pmol/mL/min, respectively. Comparisons between free (white) or encapsulated (black) forms administration were analyzed by Student’s t-test (* p < 0.05).
Figure 3
Figure 3
Quantification of εVin and εVG in plasma (a); liver (b); kidneys (c); and different white adipose tissues (d) 4 h after oral administration of 20 mg/kg bw of εVin in free (white bars) or encapsulated (black bars) forms. Bars represent concentrations expressed at pmol/mL for plasma and pmol/g of tissue for tissue + SD. Comparisons between encapsulated or free form administration were analyzed by Student’s t-test (* p < 0.05; *** p < 0.0005).
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References

    1. Lin M., Yao C.S. Studies in Natural Products Chemistry. Volume 33. Elsevier; Amsterdam, The Netherlands: 2006. Natural oligostilbenes; pp. 601–644.
    1. Pugajeva I., Perkons I., Górnaś P. Identification and Determination of Stilbenes by Q-TOF in Grape Skins, Seeds, Juice and Stems. J. Food Compos. Anal. 2018;74:44–52. doi: 10.1016/j.jfca.2018.09.007. - DOI
    1. El Khawand T., Courtois A., Valls J., Richard T., Krisa S. A Review of Dietary Stilbenes: Sources and Bioavailability. Phytochem. Rev. 2018;17:1007–1029. doi: 10.1007/s11101-018-9578-9. - DOI
    1. Vitrac X., Bornet A., Vanderlinde R., Valls J., Richard T., Delaunay J.C., Mérillon J.M., Teissédre P.L. Determination of Stilbenes (δ-Viniferin, Trans-Astringin, Trans-Piceid, Cis- and Trans-Resveratrol, ε-Viniferin) in Brazilian Wines. J. Agric. Food Chem. 2005;53:5664–5669. doi: 10.1021/jf050122g. - DOI - PubMed
    1. Zamora-Ros R., Andres-Lacueva C., Lamuela-Raventós R.M., Berenguer T., Jakszyn P., Martínez C., Sánchez M.J., Navarro C., Chirlaque M.D., Tormo M.-J., et al. Concentrations of Resveratrol and Derivatives in Foods and Estimation of Dietary Intake in a Spanish Population: European Prospective Investigation into Cancer and Nutrition (EPIC)-Spain Cohort. Br. J. Nutr. 2008;100:188–196. doi: 10.1017/S0007114507882997. - DOI - PubMed

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