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. 2022 Jul;59(7):2875-2883.
doi: 10.1007/s13197-021-05312-0. Epub 2021 Nov 29.

Development and validation of a reverse phase HPLC-DAD method for separation, detection & quantification of rutin and quercetin in buckwheat (Fagopyrum spp .)

Sabbi Jan  1 , Javaid Ahmad  1 , Mohd Masarat Dar  2 , Aijaz A Wani  1 , Inayatullah Tahir  3 , Azra N Kamili  4
Affiliations

Development and validation of a reverse phase HPLC-DAD method for separation, detection & quantification of rutin and quercetin in buckwheat (Fagopyrum spp .)

Sabbi Jan et al. J Food Sci Technol. 2022 Jul.

Abstract

Buckwheat has tremendous nutraceutical potential owing to its rutin and quercetin content. The aim of this study was to optimise and validate an analytical method for separating and quantifying these two flavonoids from it. Factors, such as range, linearity, precision, accuracy, limit of detection and limit of quantification, were evaluated for the two compounds using high performance liquid chromatography. On the basis of resolution and symmetry, mobile phase consisting of methanol and methanol:water:acetic acid in the ratio of (100:150:5), flow rate 1.3 ml/min and column temperature 30 °C were found to be optimal analytical conditions. Calibration curves exhibited good linearity with correlation coefficient of 0.995 & 0.9907 over the range 60-180 μg/ml & 2-10 μg/ml for rutin and quercetin respectively. LOD and LOQ values for rutin and quercetin were 6.36, 0.58 and 19.28, 1.77 μg/ml respectively. Recovery values of 96-100.8% confirmed that the method was accurate for rutin and quercetin analysis. This validated method was successfully used to analyse rutin and quercetin in leaves and seeds of buckwheat plant.

Keywords: Buckwheat; HPLC; Quercetin; Rutin.

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Conflict of interest statement

Conflict of interestThe authors don’t have any conflict of interest.

Figures

Fig. 1
Fig. 1
Callibration curves of standards
Fig. 2
Fig. 2
Chromatogram of standards and sample
Fig. 3
Fig. 3
UV-Visible scane of Rutin and Quercetin
See this image and copyright information in PMC

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