Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb;46(2):464-478.
doi: 10.1111/pce.14483. Epub 2022 Nov 14.

Limitation of sucrose biosynthesis shapes carbon partitioning during plant cold acclimation

Anastasia Kitashova  1 , Stephan O Adler  2 , Andreas S Richter  3 , Svenja Eberlein  1 , Dejan Dziubek  1 , Edda Klipp  2 , Thomas Nägele  1
Affiliations

Limitation of sucrose biosynthesis shapes carbon partitioning during plant cold acclimation

Anastasia Kitashova et al. Plant Cell Environ. 2023 Feb.

Abstract

Cold acclimation is a multigenic process by which many plant species increase their freezing tolerance. Stabilization of photosynthesis and carbohydrate metabolism plays a crucial role in cold acclimation. To study regulation of primary and secondary metabolism during cold acclimation of Arabidopsis thaliana, metabolic mutants with deficiencies in either starch or flavonoid metabolism were exposed to 4°C. Photosynthesis was determined together with amounts of carbohydrates, anthocyanins, organic acids and enzyme activities of the central carbohydrate metabolism. Starch deficiency was found to significantly delay soluble sugar accumulation during cold acclimation, while starch overaccumulation did not affect accumulation dynamics but resulted in lower total amounts of \sucrose and glucose. Anthocyanin amounts were lowered in both starch deficient and overaccumulating mutants. Vice versa, flavonoid deficiency did not result in a changed starch amount, which suggested a unidirectional signalling link between starch and flavonoid metabolism. Mathematical modelling of carbon metabolism indicated kinetics of sucrose biosynthesis to be limiting for carbon partitioning in leaf tissue during cold exposure. Together with cold-induced dynamics of citrate, fumarate and malate amounts, this provided evidence for a central role of sucrose phosphate synthase activity in carbon partitioning between biosynthetic and dissimilatory pathways which stabilizes photosynthesis and metabolism at low temperature.

Keywords: Arabidopsis thaliana; carbon metabolism; cold acclimation; kinetic modelling.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

REFERENCES

    1. Arrhenius, S. (1889) Über die Reaktionsgeschwindigkeit bei der Inversion von Rohrzucker durch Säuren. Zeitschrift für physikalische Chemie, 4, 226-248.
    1. Atanasov, V., Fürtauer, L. & Nägele, T. (2020) Indications for a central role of hexokinase activity in natural variation of heat acclimation in Arabidopsis thaliana. Plants, 9, 819.
    1. Austin, M.B. & Noel, J.P. (2003) The chalcone synthase superfamily of type III polyketide synthases. Natural Product Reports, 20, 79-110.
    1. Bisswanger, H. (2008) Enzyme kinetics: principles and methods. Weinheim: John Wiley & Sons.
    1. Callahan, H.S. & Pigliucci, M. (2002) Shade-induced plasticity and its ecological significance in wild populations of Arabidopsis thaliana. Ecology, 83, 1965-1980.

Publication types

LinkOut - more resources

-