NT-proBNP detection with a one-step magnetic lateral flow channel assay
- PMID: 38459191
- PMCID: PMC10973066
- DOI: 10.1007/s00216-024-05223-x
NT-proBNP detection with a one-step magnetic lateral flow channel assay
Abstract
Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a simple, mostly pain-free finger prick dramatically limits the sample size and comforts the patient. Therefore, we explored the potential of converting a conventional lateral flow assay (LFA) for a significant biomarker into a self-contained and compact polymer channel-based LFA to minimize the sample volume while maintaining the analytical merits. Our primary objective was to eliminate the use of sample-absorbing fleece and membrane materials commonly present in LFAs. Simultaneously, we concentrated on developing a ready-to-deploy one-step LFA format, characterized by dried reagents, facilitating automation and precise sample transport through a pump control system. We targeted the detection of the heart failure biomarker NT-proBNP in only 15 µL human whole blood and therefore implemented strategies that ensure highly sensitive detection. The biosensor combines streptavidin-functionalized magnetic beads (MNPs) as a 3D detection zone and fluorescence nanoparticles as signal labels in a sandwich-based immunoassay. Compared to the currently commercialized LFA, our biosensor demonstrates comparable analytical performance with only a tenth of the sample volume. With a detection limit of 43.1 pg∙mL-1 and a mean error of 18% (n ≥ 3), the biosensor offers high sensitivity and accuracy. The integration of all-dried long-term stable reagents further enhances the convenience and stability of the biosensor. This lateral flow channel platform represents a promising advancement in point-of-care diagnostics for heart failure biomarkers, offering a user-friendly and sensitive platform for rapid and reliable testing with low finger-prick blood sample volumes.
Keywords: Cardiac; Immunoassay; Lateral flow assay; Magnetic bead; One-step; Point-of-care.
© 2024. The Author(s).
Conflict of interest statement
The authors declare no competing interests. Antje J. Baeumner is the editor of this journal but was not involved in the peer review of this article.
Figures
![Fig. 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10973066/bin/216_2024_5223_Fig1_HTML.gif)
![Fig. 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10973066/bin/216_2024_5223_Fig2_HTML.gif)
![Fig. 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10973066/bin/216_2024_5223_Fig3_HTML.gif)
![Fig. 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10973066/bin/216_2024_5223_Fig4_HTML.gif)
![Fig. 5](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/10973066/bin/216_2024_5223_Fig5_HTML.gif)
Similar articles
-
Membrane-Free Lateral Flow Assay with the Active Control of Fluid Transport for Ultrasensitive Cardiac Biomarker Detection.Anal Chem. 2024 May 7;96(18):7014-7021. doi: 10.1021/acs.analchem.4c00142. Epub 2024 Apr 24. Anal Chem. 2024. PMID: 38659215 Free PMC article.
-
Sample-to-answer lateral flow assay with integrated plasma separation and NT-proBNP detection.Anal Bioanal Chem. 2024 May;416(13):3107-3115. doi: 10.1007/s00216-024-05271-3. Epub 2024 Apr 9. Anal Bioanal Chem. 2024. PMID: 38589616 Free PMC article.
-
Dry-reagent microfluidic biosensor for simple detection of NT-proBNP via Ag nanoparticles.Anal Chim Acta. 2022 Jan 25;1191:339375. doi: 10.1016/j.aca.2021.339375. Epub 2021 Dec 12. Anal Chim Acta. 2022. PMID: 35033274
-
Towards Point-of-Care Heart Failure Diagnostic Platforms: BNP and NT-proBNP Biosensors.Sensors (Basel). 2019 Nov 16;19(22):5003. doi: 10.3390/s19225003. Sensors (Basel). 2019. PMID: 31744130 Free PMC article. Review.
-
Standardization of BNP and NT-proBNP Immunoassays in Light of the Diverse and Complex Nature of Circulating BNP-Related Peptides.Adv Clin Chem. 2018;85:1-30. doi: 10.1016/bs.acc.2018.02.001. Epub 2018 Mar 3. Adv Clin Chem. 2018. PMID: 29655458 Review.
References
-
- Cardiovascular diseases. https://www.who.int/health-topics/cardiovascular-diseases. Accessed 10 Jul 2023.
-
- Cardiovascular diseases (CVDs). https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases...). Accessed 10 Jul 2023.
-
- (2022) Heart failure - what is heart failure? | NHLBI, NIH. https://www.nhlbi.nih.gov/health/heart-failure. Accessed 10 Jul 2023.
-
- Corteville DCM, Bibbins-Domingo K, Wu AHB, Ali S, Schiller NB, Whooley MA. N-terminal pro-B-type natriuretic peptide as a diagnostic test for ventricular dysfunction in patients with coronary disease: data from the heart and soul study. Arch Intern Med. 2007;167:483–489. doi: 10.1001/archinte.167.5.483. - DOI - PMC - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials