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. 2024 May 9:iyae070.
doi: 10.1093/genetics/iyae070. Online ahead of print.

UV damage induces production of mitochondrial DNA fragments with specific length profiles

Gus Waneka  1 , Joseph Stewart  2   3 , John R Anderson  4 , Wentao Li  5 , Jeffrey Wilusz  4 , Juan Lucas Argueso  2   3 , Daniel B Sloan  1   3
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UV damage induces production of mitochondrial DNA fragments with specific length profiles

Gus Waneka et al. Genetics. .

Abstract

UV light is a potent mutagen that induces bulky DNA damage in the form of cyclobutane pyrimidine dimers (CPDs). Photodamage and other bulky lesions occurring in nuclear genomes can be repaired through nucleotide excision repair (NER), where incisions on both sides of a damaged site precede the removal of a single-stranded oligonucleotide containing the damage. Mitochondrial genomes (mtDNAs) are also susceptible to damage from UV light, but current evidence suggests that the only way to eliminate bulky mtDNA damage is through mtDNA degradation. Damage-containing oligonucleotides excised during NER can be captured with anti-damage antibodies and sequenced (XR-seq) to produce high resolution maps of active repair locations following UV exposure. We analyzed previously published datasets from Arabidopsis thaliana, Saccharomyces cerevisiae, and Drosophila melanogaster to identify reads originating from the mtDNA (and plastid genome in A. thaliana). In A. thaliana and S. cerevisiae, the mtDNA-mapping reads have unique length distributions compared to the nuclear-mapping reads. The dominant fragment size was 26 nt in S. cerevisiae and 28 nt in A. thaliana with distinct secondary peaks occurring in regular intervals. These reads also show a nonrandom distribution of di-pyrimidines (the substrate for CPD formation) with TT enrichment at positions 7-8 of the reads. Therefore, UV damage to mtDNA appears to result in production of DNA fragments of characteristic lengths and positions relative to the damaged location. The mechanisms producing these fragments are unclear, but we hypothesize that they result from a previously uncharacterized DNA degradation pathway or repair mechanism in mitochondria.

Keywords: Arabidopsis thaliana; Saccharomyces cerevisiae; Mitochondrial genome (mtDNA); Photodamage; XR-seq; cyclobutane pyrimidine dimer (CPD); mtDNA degradation; nucleotide excision repair (NER).

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