TFAM is an autophagy receptor that limits inflammation by binding to cytoplasmic mitochondrial DNA
- PMID: 38783142
- DOI: 10.1038/s41556-024-01419-6
TFAM is an autophagy receptor that limits inflammation by binding to cytoplasmic mitochondrial DNA
Abstract
When cells are stressed, DNA from energy-producing mitochondria can leak out and drive inflammatory immune responses if not cleared. Cells employ a quality control system called autophagy to specifically degrade damaged components. We discovered that mitochondrial transcription factor A (TFAM)-a protein that binds mitochondrial DNA (mtDNA)-helps to eliminate leaked mtDNA by interacting with the autophagy protein LC3 through an autolysosomal pathway (we term this nucleoid-phagy). TFAM contains a molecular zip code called the LC3 interacting region (LIR) motif that enables this binding. Although mutating TFAM's LIR motif did not affect its normal mitochondrial functions, more mtDNA accumulated in the cell cytoplasm, activating inflammatory signalling pathways. Thus, TFAM mediates autophagic removal of leaked mtDNA to restrict inflammation. Identifying this mechanism advances understanding of how cells exploit autophagy machinery to selectively target and degrade inflammatory mtDNA. These findings could inform research on diseases involving mitochondrial damage and inflammation.
© 2024. The Author(s), under exclusive licence to Springer Nature Limited.
Similar articles
-
[Knockdown mitochondrial transcription factor A (TFAM) inhibits autophagy, proliferation, invasion and migration in human cervical cancer and osteosarcoma cells].Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2024 Apr;40(4):311-318. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2024. PMID: 38710515 Chinese.
-
Mitochondrial ROS promote mitochondrial dysfunction and inflammation in ischemic acute kidney injury by disrupting TFAM-mediated mtDNA maintenance.Theranostics. 2021 Jan 1;11(4):1845-1863. doi: 10.7150/thno.50905. eCollection 2021. Theranostics. 2021. PMID: 33408785 Free PMC article.
-
The mitochondrial transcription factor TFAM in neurodegeneration: emerging evidence and mechanisms.FEBS Lett. 2018 Mar;592(5):793-811. doi: 10.1002/1873-3468.12989. Epub 2018 Feb 15. FEBS Lett. 2018. PMID: 29364506 Free PMC article. Review.
-
Mitochondrial transcription factor A regulates mitochondrial transcription initiation, DNA packaging, and genome copy number.Biochim Biophys Acta. 2012 Sep-Oct;1819(9-10):921-9. doi: 10.1016/j.bbagrm.2012.03.002. Epub 2012 Mar 21. Biochim Biophys Acta. 2012. PMID: 22465614 Review.
-
Mitochondrial nucleoid and transcription factor A.Ann N Y Acad Sci. 2004 Apr;1011:61-8. doi: 10.1007/978-3-662-41088-2_7. Ann N Y Acad Sci. 2004. PMID: 15126284
Cited by
-
TCF3, TCF12 and distinct enhancers regulate oocyte transcription.Nat Cell Biol. 2024 Jun;26(6):847-848. doi: 10.1038/s41556-024-01433-8. Nat Cell Biol. 2024. PMID: 38839977 No abstract available.
-
Unfolding emergency calls stress granules to the ER.Nat Cell Biol. 2024 Jun;26(6):845-846. doi: 10.1038/s41556-024-01434-7. Nat Cell Biol. 2024. PMID: 38822214 No abstract available.
References
-
- Bonekamp, N. A. & Larsson, N. G. SnapShot: mitochondrial nucleoid. Cell 172, 388.e1 (2018). - DOI
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources