Physiological and molecular mechanism of Populus pseudo-cathayana × Populus deltoides response to Hyphantria cunea
- PMID: 38879313
- DOI: 10.1016/j.pestbp.2024.105969
Physiological and molecular mechanism of Populus pseudo-cathayana × Populus deltoides response to Hyphantria cunea
Abstract
Populus pseudo-cathayana × Populus deltoides is a crucial artificial forest tree species in Northeast China. The presence of the fall webworm (Hyphantria cunea) poses a significant threat to these poplar trees, causing substantial economic and ecological damage. This study conducted an insect-feeding experiment with fall webworm on P. pseudo-cathayana × P. deltoides, examining poplar's physiological indicators, transcriptome, and metabolome under different lengths of feeding times. Results revealed significant differences in phenylalanine ammonia-lyase activity, total phenolic content, and flavonoids at different feeding durations. Transcriptomic analysis identified numerous differentially expressed genes, including AP2/ERF, MYB, and WRKY transcription factor families exhibiting the highest expression variations. Differential metabolite analysis highlighted flavonoids and phenolic acid compounds of poplar's leaves as the most abundant in our insect-feeding experiment. Enrichment analysis revealed significant enrichment in the plant hormone signal transduction and flavonoid biosynthetic pathways. The contents of jasmonic acid and jasmonoyl-L-isoleucine increased with prolonged fall webworm feeding. Furthermore, the accumulation of dihydrokaempferol, catechin, kaempferol, and naringenin in the flavonoid biosynthesis pathway varied significantly among different samples, suggesting their crucial role in response to pest infestation. These findings provide novel insights into how poplar responds to fall webworm infestation.
Keywords: Hyphantria cunea; Jasmonic acid; Metabonomics; Populus; Transcriptomics.
Copyright © 2024. Published by Elsevier Inc.
Conflict of interest statement
Declaration of competing interest The authors declare that there are no commercial or financial relationships in the study that may be considered a potential conflict of interest.
Similar articles
-
RNA-seq of eight different poplar clones reveals conserved up-regulation of gene expression in response to insect herbivory.BMC Genomics. 2019 Aug 28;20(1):673. doi: 10.1186/s12864-019-6048-8. BMC Genomics. 2019. PMID: 31455224 Free PMC article.
-
Salicylic acid activates poplar defense against the biotrophic rust fungus Melampsora larici-populina via increased biosynthesis of catechin and proanthocyanidins.New Phytol. 2019 Jan;221(2):960-975. doi: 10.1111/nph.15396. Epub 2018 Aug 31. New Phytol. 2019. PMID: 30168132 Free PMC article.
-
Transcriptome sequencing for identification of diapause-associated genes in fall webworm, Hyphantria cunea Drury.Gene. 2018 Aug 20;668:229-236. doi: 10.1016/j.gene.2018.05.023. Epub 2018 May 25. Gene. 2018. PMID: 29758298
-
Exploring genes involved in benzoic acid biosynthesis in the Populus davidiana transcriptome and their transcriptional activity upon methyl jasmonate treatment.J Chem Ecol. 2017 Dec;43(11-12):1097-1108. doi: 10.1007/s10886-017-0903-3. Epub 2017 Nov 11. J Chem Ecol. 2017. PMID: 29129016
-
Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm.PLoS One. 2017 Jun 5;12(6):e0178754. doi: 10.1371/journal.pone.0178754. eCollection 2017. PLoS One. 2017. PMID: 28582405 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials