Switch from fetal to adult hemoglobin is associated with a change in progenitor cell population
- PMID: 6187772
- PMCID: PMC436935
- DOI: 10.1172/jci110832
Switch from fetal to adult hemoglobin is associated with a change in progenitor cell population
Abstract
To examine the switch from fetal to adult hemoglobin at the cellular level, erythroid progenitor cells from newborn infants and adults were cultured in methyl cellulose with erythropoietin. Individual erythroid colonies were labeled with [3H]leucine at various times, and globin synthesis patterns examined by gel electrophoresis and fluorography. The percent gamma- or beta-globin synthesis was determined from the total of gamma + beta, and the percent G gamma from the total of G gamma + A gamma. The nonparametric correlation coefficients of percent G gamma with percent gamma or beta were obtained. Each group of colonies at each time point was examined separately. In colonies from adult blood, the proportion of G gamma-synthesis did not correlate with the proportion of gamma-synthesis. Colonies from newborn blood fell into two groups. Those that developed from relatively mature progenitor cells, and were seen on day 14, showed a strong negative correlation of G gamma with beta-globin synthesis. However, those newborn colonies that developed from immature progenitors, and were seen later in culture (days 17 and 21), showed no correlation of G gamma with beta-synthesis. These findings are compatible with a clonal model for hemoglobin switching. Fetal progenitors, in which G gamma- and beta-syntheses are negatively correlated, are gradually replaced during ontogeny by adult progenitors. The adult progenitors produce more beta (less gamma), and the proportions of G gamma- and gamma- or beta-synthesis are not correlated.
Similar articles
-
Role of intergenic human gamma-delta-globin sequences in human hemoglobin switching and reactivation of fetal hemoglobin in adult erythroid cells.Ann N Y Acad Sci. 2005;1054:48-54. doi: 10.1196/annals.1345.057. Ann N Y Acad Sci. 2005. PMID: 16339651 Review.
-
Evidence for a clonal model for hemoglobin switching.Prog Clin Biol Res. 1983;134:431-42. Prog Clin Biol Res. 1983. PMID: 6198659
-
Control of the simian fetal hemoglobin switch at the progenitor cell level.J Clin Invest. 1981 Feb;67(2):458-66. doi: 10.1172/JCI110054. J Clin Invest. 1981. PMID: 6161945 Free PMC article.
-
Investigations of the simian ontogenic switch from fetal to adult hemoglobin at the progenitor cell level.J Clin Invest. 1986 Dec;78(6):1497-503. doi: 10.1172/JCI112741. J Clin Invest. 1986. PMID: 2431000 Free PMC article.
-
Regulation of hemoglobin synthesis during the development of the red cell (third of three parts).N Engl J Med. 1977 Dec 29;297(26):1430-6. doi: 10.1056/NEJM197712292972604. N Engl J Med. 1977. PMID: 337141 Review.
Cited by
-
A transgenic mouse model expressing exclusively human hemoglobin E: indications of a mild oxidative stress.Blood Cells Mol Dis. 2012 Feb 15;48(2):91-101. doi: 10.1016/j.bcmd.2011.12.002. Epub 2012 Jan 18. Blood Cells Mol Dis. 2012. PMID: 22260787 Free PMC article.
-
Mechanism of human Hb switching: a possible role of the kit receptor/miR 221-222 complex.Haematologica. 2010 Aug;95(8):1253-60. doi: 10.3324/haematol.2009.018259. Epub 2010 Mar 19. Haematologica. 2010. PMID: 20305142 Free PMC article.
-
Immunoblotting conditions for human hemoglobin chains.Anal Biochem. 2008 Jul 15;378(2):218-20. doi: 10.1016/j.ab.2008.04.008. Epub 2008 Apr 9. Anal Biochem. 2008. PMID: 18445469 Free PMC article.
-
Role of epigenetic modifications in normal globin gene regulation and butyrate-mediated induction of fetal hemoglobin.Blood. 2007 Nov 1;110(9):3391-7. doi: 10.1182/blood-2007-02-076091. Epub 2007 Jul 17. Blood. 2007. PMID: 17638855 Free PMC article.
-
Butyrate increases the efficiency of translation of gamma-globin mRNA.Blood. 2005 Feb 15;105(4):1807-9. doi: 10.1182/blood-2004-02-0454. Epub 2004 Oct 12. Blood. 2005. PMID: 15479724 Free PMC article. Clinical Trial.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical