A morphological study of the adipocyte precursor
- PMID: 6325721
A morphological study of the adipocyte precursor
Abstract
In order to attempt to characterize the adipocyte precursor cell in situ in developing adipose tissues from young rats, the light and electron microscopic characteristics were determined of cells in situ in these tissues, as well as in suspensions from the same tissues of cells liberated with collagenase subsequently developing to adipocytes in culture. The functional characteristics of adipose precursor cells at different stages of development in culture is known from previous work, and could therefore be added in parallel to the morphological characteristics. In order to allow comparison with fibroblasts from a site where adipocyte formation does not occur, lung-fibroblasts were also studied in parallel cultures. In culture, a rapidly dividing cell confluenced and reached full expression to adipocytes. This cell had morphological characteristics developing at the same time as early lipid accumulation making it easily distinguishable from a lung fibroblast in culture. A similar cell was found in abundance in the peripheral, non-developed part of the intact epididymal fat pad, here often surrounded by collagen. However, the early preadipocyte (without lipid droplets) and the adipoblast cannot be morphologically distinguished in situ from the fibroblast; therefore it cannot be excluded that fibroblasts and adipoblasts are the same cells in adipose tissue, possibly deriving from the pericyte.
Similar articles
-
An ultrastructural study of adipocyte precursors from epididymal fat pads of adult rats in culture.J Submicrosc Cytol. 1985 Oct;17(4):631-6. J Submicrosc Cytol. 1985. PMID: 3001331
-
S-100 protein in white preadipocytes: an immunoelectronmicroscopic study.Anat Rec. 1989 Aug;224(4):466-72. doi: 10.1002/ar.1092240403. Anat Rec. 1989. PMID: 2782627
-
The isolation and characterization of a proposed adipocyte precursor cell type from bovine subcutaneous white adipose tissue.J Dev Physiol. 1980 Oct;2(5):275-89. J Dev Physiol. 1980. PMID: 7252095
-
Influence of anatomic site and age on the replication and differentiation of rat adipocyte precursors in culture.J Clin Invest. 1983 Oct;72(4):1200-8. doi: 10.1172/JCI111075. J Clin Invest. 1983. PMID: 6630508 Free PMC article.
-
Adipocyte development in primary rat cell cultures: a scanning electron microscopy study.Anat Rec. 1986 Nov;216(3):416-22. doi: 10.1002/ar.1092160311. Anat Rec. 1986. PMID: 3789424
Cited by
-
Navigating the Adipocyte Precursor Niche: Cell-Cell Interactions, Regulatory Mechanisms and Implications for Adipose Tissue Homeostasis.J Cell Signal. 2024;5(2):65-86. doi: 10.33696/signaling.5.114. J Cell Signal. 2024. PMID: 38826152 Free PMC article.
-
Circadian mechanisms in adipose tissue bioenergetics and plasticity.Genes Dev. 2023 Jun 1;37(11-12):454-473. doi: 10.1101/gad.350759.123. Epub 2023 Jun 26. Genes Dev. 2023. PMID: 37364987 Free PMC article. Review.
-
Adipose Tissue Development Relies on Coordinated Extracellular Matrix Remodeling, Angiogenesis, and Adipogenesis.Biomedicines. 2022 Sep 8;10(9):2227. doi: 10.3390/biomedicines10092227. Biomedicines. 2022. PMID: 36140327 Free PMC article. Review.
-
Regulation of Energy Metabolism by Receptor Tyrosine Kinase Ligands.Front Physiol. 2020 Apr 21;11:354. doi: 10.3389/fphys.2020.00354. eCollection 2020. Front Physiol. 2020. PMID: 32372975 Free PMC article. Review.
-
Single cell approaches to address adipose tissue stromal cell heterogeneity.Biochem J. 2020 Feb 14;477(3):583-600. doi: 10.1042/BCJ20190467. Biochem J. 2020. PMID: 32026949 Free PMC article. Review.