Peripherin, a new member of the intermediate filament protein family
- PMID: 6399022
- DOI: 10.1159/000112360
Peripherin, a new member of the intermediate filament protein family
Abstract
Peripherin, a Triton-insoluble protein, whose distribution was found to be restricted to neurons in the rodent and human peripheral nervous system, was characterized by its electrophoretic features (isoelectric point: 5.6; molecular weight: 56,000 daltons) and by its peptidic map after limited proteolysis. Comparative peptide analysis of the 70,000-dalton subunit of neurofilaments (70K NFP), vimentin and peripherin, was performed by two different methods; limited proteolysis with Staphylococcus aureus V8 protease yields a different peptidic map for each protein; treatment with N-chlorosuccinimide, which cleaves preferentially at tryptophan residues, yields only two peptides from each protein: the size of the two fragments indicates that these proteins possess a single tryptophan residue located in the central part of the molecule. A rabbit antiserum raised against mouse peripherin decorated an intracellular filamentous network in mouse neuroblastoma NIE 115 cell line. The IgG fraction of the antiserum recognizes peripherin and the smallest subunit of the neurofilament triplet (70K NFP)--but not vimentin--whereas a monoclonal anti-70K NFP recognizes only the 70K NFP. Moreover, peripherin displays the common antigenic determinant shared by all intermediate filament proteins. Hence, we propose that peripherin represents a new member of the intermediate filament protein family, and might belong to the neurofilament class.
Similar articles
-
Phosphorylation of peripherin, an intermediate filament protein, in mouse neuroblastoma NIE 115 cell line and in sympathetic neurons.Biochem Biophys Res Commun. 1989 Apr 28;160(2):772-9. doi: 10.1016/0006-291x(89)92500-x. Biochem Biophys Res Commun. 1989. PMID: 2719695
-
Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons.J Neurosci. 2012 Jun 20;32(25):8501-8. doi: 10.1523/JNEUROSCI.1081-12.2012. J Neurosci. 2012. PMID: 22723690 Free PMC article.
-
Ontogeny of the neuronal intermediate filament protein, peripherin, in the mouse embryo.Neuroscience. 1990;36(1):217-37. doi: 10.1016/0306-4522(90)90364-a. Neuroscience. 1990. PMID: 2215919
-
Peripherin and neurofilaments: expression and role during neural development.C R Acad Sci III. 1993 Sep;316(9):1124-40. C R Acad Sci III. 1993. PMID: 8076208 Review. English, French.
-
Neuronal intermediate filaments and ALS: a new look at an old question.Biochim Biophys Acta. 2006 Nov-Dec;1762(11-12):1001-12. doi: 10.1016/j.bbadis.2006.09.003. Epub 2006 Sep 12. Biochim Biophys Acta. 2006. PMID: 17045786 Review.
Cited by
-
Deep topographic proteomics of a human brain tumour.Nat Commun. 2023 Nov 24;14(1):7710. doi: 10.1038/s41467-023-43520-8. Nat Commun. 2023. PMID: 38001067 Free PMC article.
-
Role of the Intermediate Filament Protein Peripherin in Health and Disease.Int J Mol Sci. 2022 Dec 6;23(23):15416. doi: 10.3390/ijms232315416. Int J Mol Sci. 2022. PMID: 36499746 Free PMC article. Review.
-
Posttranscriptional regulation of neurofilament proteins and tau in health and disease.Brain Res Bull. 2023 Jan;192:115-127. doi: 10.1016/j.brainresbull.2022.10.017. Epub 2022 Oct 29. Brain Res Bull. 2023. PMID: 36441047 Free PMC article. Review.
-
Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders.Hum Genomics. 2022 Jan 6;16(1):1. doi: 10.1186/s40246-021-00374-9. Hum Genomics. 2022. PMID: 34991727 Free PMC article.
-
Enterovirus-A71 exploits peripherin and Rac1 to invade the central nervous system.EMBO Rep. 2021 Jun 4;22(6):e51777. doi: 10.15252/embr.202051777. Epub 2021 Apr 19. EMBO Rep. 2021. PMID: 33871166 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
