Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I
- PMID: 728995
- DOI: 10.1016/0092-8674(78)90277-5
Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I
Abstract
A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.
Similar articles
-
Reorganization of actin in platelets stimulated by thrombin as measured by the DNase I inhibition assay.Proc Natl Acad Sci U S A. 1979 Dec;76(12):6376-80. doi: 10.1073/pnas.76.12.6376. Proc Natl Acad Sci U S A. 1979. PMID: 118466 Free PMC article.
-
The interaction of bovine pancreatic deoxyribonuclease I and skeletal muscle actin.Eur J Biochem. 1980 Mar;104(2):367-79. doi: 10.1111/j.1432-1033.1980.tb04437.x. Eur J Biochem. 1980. PMID: 6244947
-
Actin pools in extracts of teratocarcinoma cells, assayed by inhibition of DNase I.FEBS Lett. 1980 Feb 11;110(2):305-7. doi: 10.1016/0014-5793(80)80098-6. FEBS Lett. 1980. PMID: 6245925 No abstract available.
-
Actin polymerization induced by pulsed electric stimulation of bone cells in vitro.Biochim Biophys Acta. 1984 Apr 16;803(4):308-13. doi: 10.1016/0167-4889(84)90122-8. Biochim Biophys Acta. 1984. PMID: 6200146
-
Recent quantitative studies of actin filament turnover during cell locomotion.Cell Motil Cytoskeleton. 1993;25(4):309-16. doi: 10.1002/cm.970250402. Cell Motil Cytoskeleton. 1993. PMID: 8402952 Review.
Cited by
-
Mechanisms of actin disassembly and turnover.J Cell Biol. 2023 Dec 4;222(12):e202309021. doi: 10.1083/jcb.202309021. Epub 2023 Nov 10. J Cell Biol. 2023. PMID: 37948068 Free PMC article. Review.
-
Cytochalasans and Their Impact on Actin Filament Remodeling.Biomolecules. 2023 Aug 15;13(8):1247. doi: 10.3390/biom13081247. Biomolecules. 2023. PMID: 37627312 Free PMC article. Review.
-
The lens actin filament cytoskeleton: Diverse structures for complex functions.Exp Eye Res. 2017 Mar;156:58-71. doi: 10.1016/j.exer.2016.03.005. Epub 2016 Mar 10. Exp Eye Res. 2017. PMID: 26971460 Free PMC article. Review.
-
Extraction and immunochemical assays of a tubulin-like factor in cotton seedlings.Planta. 1982 Sep;154(5):402-6. doi: 10.1007/BF01267806. Planta. 1982. PMID: 24276267
-
Cofilin: a redox sensitive mediator of actin dynamics during T-cell activation and migration.Immunol Rev. 2013 Nov;256(1):30-47. doi: 10.1111/imr.12115. Immunol Rev. 2013. PMID: 24117811 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources