doi: 10.1016/0092-8674(78)90277-5.
Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I
- PMID: 728995
- DOI: 10.1016/0092-8674(78)90277-5
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Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I
Cell.
1978 Nov.
Abstract
A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.
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