Insulin-like growth factor I overexpression in human pancreatic cancer: evidence for autocrine and paracrine roles
- PMID: 7743492
Insulin-like growth factor I overexpression in human pancreatic cancer: evidence for autocrine and paracrine roles
Abstract
We assessed the potential roles of insulin-like growth factor-I (IGF-I) and the IGF-I receptor (IGF-IR) in human pancreatic cancer. IGF-I enhanced the growth of ASPC-1 and COLO-357 human pancreatic cancer cells, and this effect was significantly inhibited by a highly specific monoclonal anti-IGF-IR antibody (alpha IR3). Both cell lines expressed mRNA transcripts for IGF-IR, and basal cell growth was significantly reduced by an IGF-IR antisense oligodeoxynucleotide. IGF-I mRNA transcripts were not detected in either cell line or in two additional pancreatic cancer cell lines. In contrast, analysis of 12 pancreatic cancers revealed a 32-fold increase (P < 0.01) in IGF-I mRNA levels by comparison with the low levels observed in the normal pancreas. By in situ hybridization, IGF-I mRNA grains were present in both the cancer cells and in the surrounding connective tissue. Six of the cancers exhibited a 4.4-fold increase in IGF-IR mRNA levels. These findings suggest that IGF-I may participate in aberrant autocrine and paracrine activation of IGF-IR in pancreatic cancer in vivo.
Similar articles
-
An evaluation of the role of insulin-like growth factors (IGF) and of type-I IGF receptor signalling in hepatocarcinogenesis and in the resistance of hepatocarcinoma cells against drug-induced apoptosis.Biochem Pharmacol. 2004 Sep 15;68(6):1003-15. doi: 10.1016/j.bcp.2004.05.029. Biochem Pharmacol. 2004. PMID: 15313394 Review.
-
Insulin-like growth factor family in malignant haemangiopericytomas: the expression and role of insulin-like growth factor I receptor.J Pathol. 1999 May;188(1):69-75. doi: 10.1002/(SICI)1096-9896(199905)188:1<69::AID-PATH329>3.0.CO;2-P. J Pathol. 1999. PMID: 10398143
-
The role of insulin-like growth factor I and its receptor in cell growth, transformation, apoptosis, and chemoresistance in solid tumors.J Cancer Res Clin Oncol. 1999;125(3-4):166-73. doi: 10.1007/s004320050259. J Cancer Res Clin Oncol. 1999. PMID: 10235470 Review.
-
Fibroblast growth factor-5 stimulates mitogenic signaling and is overexpressed in human pancreatic cancer: evidence for autocrine and paracrine actions.Oncogene. 1997 Sep 18;15(12):1417-24. doi: 10.1038/sj.onc.1201307. Oncogene. 1997. PMID: 9333017
-
Overexpression of the insulin-like growth factor-1 receptor and autocrine stimulation in human cervical cancer cells.Cancer Res. 1996 Apr 15;56(8):1761-5. Cancer Res. 1996. PMID: 8620490
Cited by
-
Down-regulation of AKT proteins slows the growth of mutant-KRAS pancreatic tumors.bioRxiv [Preprint]. 2024 May 5:2024.05.03.592345. doi: 10.1101/2024.05.03.592345. bioRxiv. 2024. Update in: Cells. 2024 Jun 19;13(12):1061. doi: 10.3390/cells13121061. PMID: 38746217 Free PMC article. Updated. Preprint.
-
Lifestyle Medicine for the Prevention and Treatment of Pancreatitis and Pancreatic Cancer.Diagnostics (Basel). 2024 Mar 14;14(6):614. doi: 10.3390/diagnostics14060614. Diagnostics (Basel). 2024. PMID: 38535034 Free PMC article. Review.
-
Sugar sweetened beverages, natural fruit juices, and cancer: what we know and what still needs to be assessed.Front Nutr. 2023 Dec 21;10:1301335. doi: 10.3389/fnut.2023.1301335. eCollection 2023. Front Nutr. 2023. PMID: 38178975 Free PMC article. Review.
-
A hierarchical prognostic model for Co-diabetes pancreatic adenocarcinoma.Heliyon. 2023 Nov 4;9(11):e21642. doi: 10.1016/j.heliyon.2023.e21642. eCollection 2023 Nov. Heliyon. 2023. PMID: 38027595 Free PMC article.
-
Targeting the IGF-Axis Potentiates Immunotherapy for Pancreatic Ductal Adenocarcinoma Liver Metastases by Altering the Immunosuppressive Microenvironment.Mol Cancer Ther. 2021 Dec;20(12):2469-2482. doi: 10.1158/1535-7163.MCT-20-0144. Epub 2021 Sep 22. Mol Cancer Ther. 2021. PMID: 34552012 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources
Medical