Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription
- PMID: 7958900
- DOI: 10.1101/gad.8.19.2336
Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription
Abstract
Human TFIIA activity is composed of three subunits (alpha, beta, gamma). Here we report the isolation of a human cDNA clone encoding the gamma-subunit and the reconstitution of TFIIA activity from recombinant polypeptides (holo-TFIIA). Protein-protein interaction analysis established that the beta and gamma subunits of TFIIA interact with the TBP component of TFIID. The alpha-subunit is recruited into the complex by association with the gamma-subunit. Functional studies indicate that recombinant TFIIA stimulates basal TFIID-dependent transcription but is without effect on TBP-dependent transcription. Our studies indicate that TFIIA not only functions by physically removing negative components present in TFIID (antirepression), as demonstrated previously, but that it can stimulate basal transcription through components of the TFIID complex. Holo-TFIIA also stimulated activation of transcription in vitro as well as in vivo in transfected HeLa cells.
Similar articles
-
Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.Genes Dev. 1994 Oct 1;8(19):2324-35. doi: 10.1101/gad.8.19.2324. Genes Dev. 1994. PMID: 7958899
-
Taf(II) 250 phosphorylates human transcription factor IIA on serine residues important for TBP binding and transcription activity.J Biol Chem. 2001 May 11;276(19):15886-92. doi: 10.1074/jbc.M009385200. Epub 2001 Feb 20. J Biol Chem. 2001. PMID: 11278496
-
Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription.Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3313-7. doi: 10.1073/pnas.92.8.3313. Proc Natl Acad Sci U S A. 1995. PMID: 7724559 Free PMC article.
-
Mechanisms of viral activators.Cold Spring Harb Symp Quant Biol. 1998;63:243-52. doi: 10.1101/sqb.1998.63.243. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384288 Review.
-
TBP-associated factors (TAFIIs): multiple, selective transcriptional mediators in common complexes.Trends Biochem Sci. 2000 Feb;25(2):59-63. doi: 10.1016/s0968-0004(99)01527-3. Trends Biochem Sci. 2000. PMID: 10664584 Review.
Cited by
-
Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1.Curr Genet. 2023 Dec;69(4-6):289-300. doi: 10.1007/s00294-023-01277-z. Epub 2023 Nov 10. Curr Genet. 2023. PMID: 37947853 Free PMC article.
-
Requirements for RNA polymerase II preinitiation complex formation in vivo.Elife. 2019 Jan 25;8:e43654. doi: 10.7554/eLife.43654. Elife. 2019. PMID: 30681409 Free PMC article.
-
Disease-relevant signalling-pathways in head and neck cancer: Taspase1's proteolytic activity fine-tunes TFIIA function.Sci Rep. 2017 Nov 2;7(1):14937. doi: 10.1038/s41598-017-14814-x. Sci Rep. 2017. PMID: 29097782 Free PMC article.
-
TBP-like protein (TLP) interferes with Taspase1-mediated processing of TFIIA and represses TATA box gene expression.Nucleic Acids Res. 2015 Jul 27;43(13):6285-98. doi: 10.1093/nar/gkv576. Epub 2015 Jun 1. Nucleic Acids Res. 2015. PMID: 26038314 Free PMC article.
-
Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.Transcription. 2015;6(2):21-32. doi: 10.1080/21541264.2015.1052178. Transcription. 2015. PMID: 25996597 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases