Localization of cannabinoid receptor mRNA in rat brain
- PMID: 8440779
- DOI: 10.1002/cne.903270406
Localization of cannabinoid receptor mRNA in rat brain
Abstract
Cannabinoid receptor mRNA was localized in adult rat brain by 35S-tailed oligonucleotide probes and in situ hybridization histochemistry. Labelling is described as uniform or non-uniform depending on the relative intensities of individual cells expressing cannabinoid receptor mRNA within a given region or nucleus. Uniform labelling was found in the hypothalamus, thalamus, basal ganglia, cerebellum and brainstem. Non-uniform labelling that resulted from the presence of cells displaying two easily distinguishable intensities of hybridization signals was observed in several regions and nuclei in the forebrain (cerebral cortex, hippocampus, amygdala, certain olfactory structures). Olfactory-associated structures, basal ganglia, hippocampus, and cerebellar cortex displayed the heaviest amounts of labelling. Many regions that displayed cannabinoid receptor mRNA could reasonably be identified as sources for cannabinoid receptors on the basis of well documented hodologic data. Other sites that were also clearly labelled could not be assigned as logical sources of cannabinoid receptors. The localization of cannabinoid receptor mRNA indicates that sensory, motor, cognitive, limbic, and autonomic systems should all be influenced by the activation of this receptor by either exogenous cannabimimetics, including marijuana, or the yet unknown endogenous "cannabinoid" ligand.
Similar articles
-
Cannabinoid CB2 receptors: immunohistochemical localization in rat brain.Brain Res. 2006 Feb 3;1071(1):10-23. doi: 10.1016/j.brainres.2005.11.035. Epub 2006 Feb 9. Brain Res. 2006. PMID: 16472786
-
Striatal cannabinoid CB1 receptor mRNA expression is decreased in the reserpine-treated rat model of Parkinson's disease.Exp Neurol. 2001 Jun;169(2):400-6. doi: 10.1006/exnr.2001.7649. Exp Neurol. 2001. PMID: 11358453
-
Analysis of cannabinoid receptor binding and mRNA expression and endogenous cannabinoid contents in the developing rat brain during late gestation and early postnatal period.Synapse. 1999 Sep 1;33(3):181-91. doi: 10.1002/(SICI)1098-2396(19990901)33:3<181::AID-SYN3>3.0.CO;2-R. Synapse. 1999. PMID: 10420166
-
Distributions of leptin receptor mRNA isoforms in the rat brain.J Comp Neurol. 1998 Jun 15;395(4):535-47. J Comp Neurol. 1998. PMID: 9619505
-
Cannabinoid receptors: which cells, where, how, and why?NIDA Res Monogr. 1992;126:48-56. NIDA Res Monogr. 1992. PMID: 1491718 Review.
Cited by
-
Cannabinoid type-1 receptor signaling in dopaminergic Engrailed-1 expressing neurons modulates motivation and depressive-like behavior.Front Mol Neurosci. 2024 Apr 4;17:1379889. doi: 10.3389/fnmol.2024.1379889. eCollection 2024. Front Mol Neurosci. 2024. PMID: 38660383 Free PMC article.
-
Selective alterations of endocannabinoid system genes expression in obsessive compulsive disorder.Transl Psychiatry. 2024 Feb 26;14(1):118. doi: 10.1038/s41398-024-02829-8. Transl Psychiatry. 2024. PMID: 38409080 Free PMC article.
-
Imaging and Genetic Tools for the Investigation of the Endocannabinoid System in the CNS.Int J Mol Sci. 2023 Oct 31;24(21):15829. doi: 10.3390/ijms242115829. Int J Mol Sci. 2023. PMID: 37958825 Free PMC article. Review.
-
Cannabis Use and Associated Gastrointestinal Disorders: A Literature Review.Cureus. 2023 Jul 13;15(7):e41825. doi: 10.7759/cureus.41825. eCollection 2023 Jul. Cureus. 2023. PMID: 37575784 Free PMC article. Review.
-
Genetic Insights into the Molecular Pathophysiology of Depression in Parkinson's Disease.Medicina (Kaunas). 2023 Jun 13;59(6):1138. doi: 10.3390/medicina59061138. Medicina (Kaunas). 2023. PMID: 37374342 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources