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. 1996 Nov 29;87(5):917-27.
doi: 10.1016/s0092-8674(00)81998-4.

High frequency retrotransposition in cultured mammalian cells

J V Moran  1 , S E Holmes, T P Naas, R J DeBerardinis, J D Boeke, H H Kazazian Jr
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Free article

High frequency retrotransposition in cultured mammalian cells

J V Moran et al. Cell. .
Free article

Abstract

We previously isolated two human L1 elements (L1.2 and LRE2) as the progenitors of disease-producing insertions. Here, we show these elements can actively retrotranspose in cultured mammalian cells. When stably expressed from an episome in HeLa cells, both elements retrotransposed into a variety of chromosomal locations at a high frequency. The retrotransposed products resembled endogenous L1 insertions, since they were variably 5' truncated, ended in poly(A) tracts, and were flanked by target-site duplications or short deletions. Point mutations in conserved domains of the L1.2-encoded proteins reduced retrotransposition by 100- to 1000-fold. Remarkably, L1.2 also retrotransposed in a mouse cell line, suggesting a potential role for L1-based vectors in random insertional mutagenesis.

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