Diet-induced muscle insulin resistance in rats is ameliorated by acute dietary lipid withdrawal or a single bout of exercise: parallel relationship between insulin stimulation of glucose uptake and suppression of long-chain fatty acyl-CoA
- PMID: 9392490
- DOI: 10.2337/diab.46.12.2022
Diet-induced muscle insulin resistance in rats is ameliorated by acute dietary lipid withdrawal or a single bout of exercise: parallel relationship between insulin stimulation of glucose uptake and suppression of long-chain fatty acyl-CoA
Abstract
Chronic high-fat feeding in rats induces profound whole-body insulin resistance, mainly due to effects in oxidative skeletal muscle. The mechanisms of this reaction remain unclear, but local lipid availability has been implicated. The aim of this study was to examine the influence of three short-term physiological manipulations intended to lower muscle lipid availability on insulin sensitivity in high-fat-fed rats. Adult male Wistar rats fed a high-fat diet for 3 weeks were divided into four groups the day before the study: one group was fed the normal daily high-fat meal (FM); another group was fed an isocaloric low-fat high-glucose meal (GM); a third group was fasted overnight (NM); and a fourth group underwent a single bout of exercise (2-h swim), then were fed the normal high-fat meal (EX). In vivo insulin action was assessed using the hyperinsulinemic glucose clamp (plasma insulin 745 pmol/l, glucose 7.2 mmol/l). Prior exercise, a single low-fat meal, or fasting all significantly increased insulin-stimulated glucose utilization, estimated at either the whole-body level (P < 0.01 vs. FM) or in red quadriceps muscle (EX 18.2, GM 28.1, and NM 19.3 vs. FM 12.6 +/- 1.1 micromol x 100 g(-1) x min(-1); P < 0.05), as well as increased insulin suppressibility of muscle total long-chain fatty acyl-CoA (LC-CoA), the metabolically available form of fatty acid (EX 24.0, GM 15.5, and NM 30.6 vs. FM 45.4 nmol/g; P < 0.05). There was a strong inverse correlation between glucose uptake and LC-CoA in red quadriceps during the clamp (r = -0.7, P = 0.001). Muscle triglyceride was significantly reduced by short-term dietary lipid withdrawal (GM -22 and NM -24% vs. FM; P < 0.01), but not prior exercise. We concluded that muscle insulin resistance induced by high-fat feeding is readily ameliorated by three independent, short-term physiological manipulations. The data suggest that insulin resistance is an important factor in the elevated muscle lipid availability induced by chronic high-fat feeding.
Similar articles
-
Reduced malonyl-CoA content in recovery from exercise correlates with improved insulin-stimulated glucose uptake in human skeletal muscle.Am J Physiol Endocrinol Metab. 2009 Apr;296(4):E787-95. doi: 10.1152/ajpendo.90556.2008. Epub 2009 Feb 3. Am J Physiol Endocrinol Metab. 2009. PMID: 19190265 Clinical Trial.
-
Triglycerides, fatty acids and insulin resistance--hyperinsulinemia.Exp Clin Endocrinol Diabetes. 2001;109(4):S516-26. doi: 10.1055/s-2001-15114. Exp Clin Endocrinol Diabetes. 2001. PMID: 11453039 Review.
-
Acute reversal of lipid-induced muscle insulin resistance is associated with rapid alteration in PKC-theta localization.Am J Physiol Endocrinol Metab. 2000 Nov;279(5):E1196-201. doi: 10.1152/ajpendo.2000.279.5.E1196. Am J Physiol Endocrinol Metab. 2000. PMID: 11052977
-
Malonyl CoA, long chain fatty acyl CoA and insulin resistance in skeletal muscle.J Basic Clin Physiol Pharmacol. 1998;9(2-4):295-308. doi: 10.1515/jbcpp.1998.9.2-4.295. J Basic Clin Physiol Pharmacol. 1998. PMID: 10212840 Review.
-
Mechanisms of liver and muscle insulin resistance induced by chronic high-fat feeding.Diabetes. 1997 Nov;46(11):1768-74. doi: 10.2337/diab.46.11.1768. Diabetes. 1997. PMID: 9356024
Cited by
-
DIETARY REGULATION OF SILENT SYNAPSES IN THE DORSOLATERAL STRIATUM.bioRxiv [Preprint]. 2024 Mar 27:2024.03.24.586457. doi: 10.1101/2024.03.24.586457. bioRxiv. 2024. PMID: 38585967 Free PMC article. Preprint.
-
The Influence of Physical Activity on the Bioactive Lipids Metabolism in Obesity-Induced Muscle Insulin Resistance.Biomolecules. 2020 Dec 12;10(12):1665. doi: 10.3390/biom10121665. Biomolecules. 2020. PMID: 33322719 Free PMC article. Review.
-
Can antioxidants be effective therapeutics for type 2 diabetes?Yeungnam Univ J Med. 2021 Apr;38(2):83-94. doi: 10.12701/yujm.2020.00563. Epub 2020 Oct 8. Yeungnam Univ J Med. 2021. PMID: 33028055 Free PMC article.
-
The Complex Interactions Between Obesity, Metabolism and the Brain.Front Neurosci. 2019 May 24;13:513. doi: 10.3389/fnins.2019.00513. eCollection 2019. Front Neurosci. 2019. PMID: 31178685 Free PMC article. Review.
-
Atlas of Circadian Metabolism Reveals System-wide Coordination and Communication between Clocks.Cell. 2018 Sep 6;174(6):1571-1585.e11. doi: 10.1016/j.cell.2018.08.042. Cell. 2018. PMID: 30193114 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous