Differential regulation of mouse uncoupling proteins among brown adipose tissue, white adipose tissue, and skeletal muscle in chronic beta 3 adrenergic receptor agonist treatment
- PMID: 9875224
- DOI: 10.1006/bbrc.1998.9746
Differential regulation of mouse uncoupling proteins among brown adipose tissue, white adipose tissue, and skeletal muscle in chronic beta 3 adrenergic receptor agonist treatment
Abstract
Uncoupling proteins (UCPs) are inner mitochondrial membrane transporters that dissipate the proton gradient, releasing stored energy as heat, without coupling to other energy-consuming processes. Therefore, the UCPs are thought to be important determinants of the metabolic efficiency. To elucidate relationships between the UCPs expressions and insulin sensitivity improvement, we treated KK-Ay mice with beta 3 adrenergic receptor agonist for 21 days and examined the changes of the UCPs mRNA expressions in various tissues. Chronic treatment of a specific beta 3 adrenergic receptor agonist, CL316,243 (0.2 mg/kg body weight/day s.c.) markedly increased the expressions of uncoupling protein 1 (UCP1), uncoupling protein 2 (UCP2), and uncoupling protein 3 (UCP3) by 14-fold, 6-fold, and 16-fold, respectively, in the brown adipose tissue (BAT). The UCP1 and UCP3 mRNA expressions in the white adipose tissue (WAT) were also increased by 12-fold and 9-fold, respectively, but the UCP2 mRNA expression was not changed in this tissue. Interestingly, the UCP2 and UCP3 mRNA expressions were strikingly decreased in the skeletal muscle and heart. Particularly, the UCP3 mRNA expression level in the skeletal muscle was dropped to 10% of that of the saline-treated control mice, indicating that the UCPs mRNA expressions are regulated in tissue-specific ways. The concentrations of plasma insulin and circulating free fatty acid (FFA) were significantly decreased, suggesting that they correlate with the reductions of the UCP2 and UCP3 mRNA expressions in the skeletal muscle and heart. It has been thought that the UCP1 and UCP3 mRNA expressions in the BAT and WAT are mainly controlled by the hypothalamus via the sympathetic nervous system, while the levels of insulin, FFA or both may play important roles in the control of the UCP2 and UCP3 mRNA expressions in the skeletal muscle an heart.
Similar articles
-
Effects of growth hormone (GH) on mRNA levels of uncoupling proteins 1, 2, and 3 in brown and white adipose tissues and skeletal muscle in obese mice.Horm Metab Res. 2004 Sep;36(9):607-13. doi: 10.1055/s-2004-825905. Horm Metab Res. 2004. PMID: 15486811
-
Regulation of UCP1, UCP2, and UCP3 mRNA expression in brown adipose tissue, white adipose tissue, and skeletal muscle in rats by estrogen.Biochem Biophys Res Commun. 2001 Oct 19;288(1):191-7. doi: 10.1006/bbrc.2001.5763. Biochem Biophys Res Commun. 2001. PMID: 11594772
-
UCP1: the original uncoupling protein--and perhaps the only one? New perspectives on UCP1, UCP2, and UCP3 in the light of the bioenergetics of the UCP1-ablated mice.J Bioenerg Biomembr. 1999 Oct;31(5):475-91. doi: 10.1023/a:1005400507802. J Bioenerg Biomembr. 1999. PMID: 10653476 Review.
-
Human uncoupling proteins and obesity.Obes Res. 1999 Jan;7(1):97-105. doi: 10.1002/j.1550-8528.1999.tb00396.x. Obes Res. 1999. PMID: 10023736 Review.
-
Differential regulation of uncoupling proteins by chronic treatments with beta 3-adrenergic agonist BRL 35135 and metformin in obese fa/fa Zucker rats.Biochem Biophys Res Commun. 1998 May 29;246(3):899-904. doi: 10.1006/bbrc.1998.8721. Biochem Biophys Res Commun. 1998. PMID: 9618309
Cited by
-
Effects of Combined Oxytocin and Beta-3 Receptor Agonist (CL 316243) Treatment on Body Weight and Adiposity in Male Diet-Induced Obese Rats.Front Physiol. 2021 Sep 8;12:725912. doi: 10.3389/fphys.2021.725912. eCollection 2021. Front Physiol. 2021. PMID: 34566687 Free PMC article.
-
Neuroendocrine Regulation of Energy Metabolism Involving Different Types of Adipose Tissues.Int J Mol Sci. 2019 Jun 1;20(11):2707. doi: 10.3390/ijms20112707. Int J Mol Sci. 2019. PMID: 31159462 Free PMC article. Review.
-
Evaluation of Glucose Uptake and Uncoupling Protein 1 Activity in Adipose Tissue of Diabetic Mice upon β-Adrenergic Stimulation.Mol Imaging Biol. 2019 Apr;21(2):249-256. doi: 10.1007/s11307-018-1251-6. Mol Imaging Biol. 2019. PMID: 29987618
-
Zinc-α2-Glycoprotein Is Associated with Obesity in Chinese People and HFD-Induced Obese Mice.Front Physiol. 2018 Feb 7;9:62. doi: 10.3389/fphys.2018.00062. eCollection 2018. Front Physiol. 2018. PMID: 29467670 Free PMC article.
-
Proteomic identification of fat-browning markers in cultured white adipocytes treated with curcumin.Mol Cell Biochem. 2016 Apr;415(1-2):51-66. doi: 10.1007/s11010-016-2676-3. Epub 2016 Feb 25. Mol Cell Biochem. 2016. PMID: 26915100
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials